STAT3 anticorps (Internal Region)
Aperçu rapide pour STAT3 anticorps (Internal Region) (ABIN185692)
Antigène
Voir toutes STAT3 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
Classe de qualité
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Épitope
- Internal Region
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Fonction
- STAT3
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Séquence
- CRPESQEHPE ADP
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Specificité
- This antibody is expected to recognise all three reported isoforms (NP_644805.1, NP_003141.2 and NP_998827.1).
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Réactivité croisée
- Chien, Humain, Souris, Rat
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Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Immunogène
- Peptide with sequence CRPESQEHPEADP, from the internal region of the protein sequence according to NP_644805.1, NP_003141.2, NP_998827.1.
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Isotype
- IgG
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Indications d'application
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Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.
Western Blot: Approx 85 kDa band observed in lysates of cell line LNCaP, and approx. 90 kDa in Mouse Heart lysates (calculated MW of 83.1 kDa according to Human NP_998827.1 and 88.1 kDa according to Mouse : NP_998824.1). Recommended concentration: 0.5-3 μg/mL
Peptide ELISA: antibody detection limit dilution 1:16000.
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Commentaires
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Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Minimize freezing and thawing.
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Stock
- -20 °C
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Stockage commentaire
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))
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Autre désignation
- STAT3
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Sujet
- STAT3, signal transducer and activator of transcription 3 (acute-phase response factor), APRF, FLJ20882, MGC16063 , DNA-binding protein APRF, acute-phase response factor, signal transducer and activator of transcription 3
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ID gène
- 6774, 20848, 25125
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NCBI Accession
- NP_644805, NP_003141, NP_998827
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Pathways
- Signalistation JAK/STAT, Signalisation RTK, Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Stem Cell Maintenance, Hepatitis C, Protein targeting to Nucleus, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor
Antigène
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