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STAT3 anticorps (C-Term)

Name: STAT3 anticorps (C-Term)
Brand: antibodies-online
SKU: ABIN184593
Availability: InStock

Cet anticorps anti-STAT3 Polyclonal Chèvre (ABIN184593) détecte spécifiquement STAT3 dans IHC, ELISA, IF et FACS. L’anticorps est réactif avec des échantillons de Humain et Souris.

ABIN184593 (4µg/ml) staining of paraffin embedded Mouse Thymus. (STAT3 anticorps (C-Term))

IHC

ABIN184593 overnight staining at 10ug/ml on 4%PFA-fixed mouse thymus. (STAT3 anticorps (C-Term))

IHC

ABIN184593 (1µg/ml) staining of NIH3T3 lysate (35µg protein in RIPA buffer). (STAT3 anticorps (C-Term))

ABIN184593 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0. (STAT3 anticorps (C-Term))

FACS

ABIN184593 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. (STAT3 anticorps (C-Term))

ABIN184593 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. (STAT3 anticorps (C-Term))

IHC

7 images

N° du produit ABIN184593

488,25 €

Plus frais de livraison 40,00 € et TVA

100 μg

Destination: France

Envoi sous 5 à 8 jours ouvrables

Aperçu rapide pour STAT3 anticorps (C-Term) (ABIN184593)

Antigène

Voir toutes STAT3 Anticorps

STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))

Reactivité

Humain, Souris

Hôte

Chèvre

Clonalité

Polyclonal

Conjugué

Cet anticorp STAT3 est non-conjugé

Application

Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Classe de qualité

Verified

Discover our top product STAT3 Anticorps primaire

Épitope
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C-Term

Fonction

STAT3 (isoform 1 and 2)

Séquence

DMELTSECAT SPM

Specificité

This antibody is expected to recognise isoforms 1 and 2 (as represented by NP_644805.1 and NP_003141.2 respectively)

Réactivité croisée

Boeuf (Vache), Humain, Souris, Rat

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène

Peptide with sequence DMELTSECATSPM, from the C Terminus of the protein sequence according to NP_003141.2, NP_644805.1.

Isotype

IgG
anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (C-Term) antibody
KO Validated STAT3 Reactivité: Humain WB, IF, ICC, IHC (p) Hôte: Lapin Polyclonal unconjugated

anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (AA 640-770) antibody
STAT3 Reactivité: Humain WB, IHC, IF Hôte: Lapin Polyclonal unconjugated

anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (pTyr705) antibody
STAT3 Reactivité: Humain, Souris, Rat WB, IHC, ELISA, IP, IF, ICC Hôte: Lapin Polyclonal unconjugated

anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (pSer727) antibody
STAT3 Reactivité: Humain, Souris, Rat WB, IHC, ELISA, IP, IF, ICC Hôte: Lapin Polyclonal unconjugated

anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (C-Term) antibody
STAT3 Reactivité: Humain, Souris, Rat WB, IHC, ELISA, IF, ICC Hôte: Lapin Polyclonal unconjugated

anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (pSer727) antibody
STAT3 Reactivité: Humain, Souris, Rat WB, IHC (p), FACS, IF (cc) Hôte: Lapin Monoclonal 4G1 unconjugated

anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (AA 670-769) antibody
STAT3 Reactivité: Humain WB, ELISA, IF, PLA Hôte: Souris Monoclonal 4D6 unconjugated

anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (AA 233-330) antibody
STAT3 Reactivité: Humain, Souris, Rat, Lapin WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (pTyr705) antibody
STAT3 Reactivité: Humain, Souris, Rat, Porc, Lapin, Poulet WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (pSer727) antibody
STAT3 Reactivité: Humain, Souris, Rat, Singe WB, IHC, ELISA, IP, IF Hôte: Lapin Polyclonal unconjugated

Indications d'application

Immunohistochemistry: In paraffin embedded Mouse Thymus shows preferential staining of medula over cortex. Recommended concentration: 4-6 μg/mL.
Western Blot: Preliminary testing showed a band at approx 80-85 kDa in A431 nuclear cell lysate after 2 μg/mL antibody staining (calculated MW of 80 kDa according to NP_003141.2, and NP_644805.1). Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:8000.

Commentaires

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and A431 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

Restrictions

For Research Use only
Format

Liquid

Concentration

0.5 mg/mL

Buffer

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation

Minimize freezing and thawing.

Stock

-20 °C

Stockage commentaire

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Antigène

STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))

Autre désignation

STAT3

Sujet

STAT3, signal transducer and activator of transcription 3 (acute-phase response factor), APRF, DNA-binding protein APRF, acute-phase response factor, FLJ20882, MGC16063, HIES

ID gène

6774, 20848, 25125

NCBI Accession

NP_003141, NP_644805

Pathways

Signalistation JAK/STAT, Signalisation RTK, Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Stem Cell Maintenance, Hepatitis C, Protein targeting to Nucleus, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor