E-cadherin anticorps
(4 références)Aperçu rapide pour E-cadherin anticorps (ABIN459320)
Antigène
Voir toutes E-cadherin (CDH1) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Specificité
- 5H9 recognizes both the 120 kD E-cadherin and its 80 kD trypsin-resistant extracellular part.
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Purification
- Affinity purified
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Immunogène
- 5H9 is a mouse monoclonal IgG1 antibody obtained by fusion of P3-X63-Ag 8,653 mouse myeloma cells with spleen cells from a BABL/c mouse immunized with affinity purified 80 kD extracellular fragments of E-cadherin derived from tryptic digestion of A-431 human vulva carcinoma cells.
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Isotype
- IgG1
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anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 401-500) antibody
CDH1 Reactivité: Humain, Souris, Rat WB, FACS, ELISA, ICC, IHC (p), IF (p), IHC (fro), IF (cc) Hôte: Lapin Polyclonal unconjugated
anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) antibodyKO Validated CDH1 Reactivité: Humain WB, IF, IP, ICC, IHC (p), IHC (wm) Hôte: Lapin Polyclonal unconjugated
anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 381-480) antibodyCDH1 Reactivité: Humain WB, ELISA, IHC (p), PLA Hôte: Souris Monoclonal 3F4 unconjugated
anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (C-Term) antibodyCDH1 Reactivité: Humain, Souris, Rat WB, IHC, IF, ELISA, ICC Hôte: Lapin Polyclonal unconjugated
anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) antibodyCDH1 Reactivité: Humain, Souris, Rat, Porc, Singe, Poulet, Chien, Boeuf (Vache), Cheval, Lapin, Cobaye, Avian, Cat, Âne, Chévre, Hamster, Mouton WB, IF, IHC (p), IHC (fro) Hôte: Chèvre Polyclonal unconjugated
anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) antibodyCDH1 Reactivité: Humain, Souris, Rat, Porc, Singe, Poulet, Chien, Boeuf (Vache), Cheval, Lapin, Cobaye, Avian, Cat, Âne, Chévre, Hamster, Mouton WB, IF, IHC (p), IHC (fro) Hôte: Chèvre Polyclonal unconjugated
anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 155-707) antibodyCDH1 Reactivité: Humain, Souris WB, IHC, FACS, IP, ICC, IF (p) Hôte: Lapin Monoclonal BLR088G unconjugated Recombinant Antibody
anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 810-890) antibodyCDH1 Reactivité: Humain, Souris, Rat WB, IHC, IF, ELISA Hôte: Lapin Polyclonal unconjugated
anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) antibodyCDH1 Reactivité: Humain, Souris WB, IHC, FACS, IF Hôte: Souris Monoclonal CDH1-1525 unconjugated
anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 375-631) antibodyCDH1 Reactivité: Porc WB, IHC, IP, ICC Hôte: Lapin Polyclonal unconjugated
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Indications d'application
- 5H9 is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen and paraffin embedded tissues. For frozen tissues use a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2. For paraffin embedded tissues use a pretreatment step of 15 minutes incubation in TRIS-EDTA buffer pH 9 in a microwave. Recommended dilution Optimal antibody dilution should be determined by titration, recommended range is 1:50 – 1:100 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:500 for immunoblotting applications.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1mg/ml
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Buffer
- Each vial contains 100 ml of 1 mg/ml purified antibody in PBS containing 0.09% sodium azide.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C
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: "E-Cadherin in human brain tumours: loss of immunoreactivity in malignant meningiomas." dans: Virchows Archiv : an international journal of pathology, Vol. 432, Issue 2, pp. 163-7, (1998) (PubMed).
: "Prognostic value of E-cadherin expression in 413 gastric carcinomas." dans: International journal of cancer. Journal international du cancer, Vol. 69, Issue 3, pp. 184-9, (1996) (PubMed).
: "Differential loss of E-cadherin expression in infiltrating ductal and lobular breast carcinomas." dans: The American journal of pathology, Vol. 143, Issue 6, pp. 1731-42, (1994) (PubMed).
: "E-cadherin-mediated cell-cell adhesion prevents invasiveness of human carcinoma cells." dans: The Journal of cell biology, Vol. 113, Issue 1, pp. 173-85, (1991) (PubMed).
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- E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))
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Autre désignation
- E-Cadherin/Cadherin-1
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Sujet
- Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4), whereas kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. One of the best characterized cadherins is E-cadherin, a 120 kD transmembrane glycoprotein consisting of an 80 kD extracellular and a 40 kD transmembrane and cytoplasmic part. The extracellular domains of E-cadherin are responsible for calcium binding which allows for homophilic interaction with other E-cadherin molecules on the same cell and neighbouring cells. In addition, E-cadherin can interact heterophilically with integrin E7. The cytoplasmic domain of E-cadherin is linked to the actin cytoskeleton through the associated cytoplasmic catenin proteins, thus establishing a complex localized to adherens junctions. In carcinomas E-cadherin is frequently downregulated, which is consistent with its function of an invasion suppressor in normal epithelia.
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Pathways
- Signalisation WNT, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation
Antigène
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