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LAT2 anticorps (AA 91-244)

Name: LAT2 anticorps (AA 91-244)
SKU: ABIN120727
Availability: InStock

Cet anticorps Souris Monoclonal détecte spécifiquement LAT2 dans WB, IP, FACS et IF. Il présente une réactivité avec des échantillons de Humain et Souris. Il a été cité dans 3+ publications.

(3 références)

N° du produit ABIN120727

544,62 €

Plus frais de livraison 40,00 € et TVA

0.1 mg

Destination: France

Envoi sous 17 jours ouvrables

Aperçu rapide pour LAT2 anticorps (AA 91-244) (ABIN120727)

Antigène

Voir toutes LAT2 Anticorps

LAT2 (Linker For Activation of T Cells Family, Member 2 (LAT2))

Reactivité

Humain, Souris

Hôte

Souris

Clonalité

Monoclonal

Conjugué

Cet anticorp LAT2 est non-conjugé

Application

Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone

NAP-07

Discover our top product LAT2 Anticorps primaire

Épitope
8

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AA 91-244

Specificité

The antibody reacts with Non-T cell activation linker (NTAL), also known as LAB (linker of activated B cells), a 25 - 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B lymphocytes, NK cells and myeloid cells.

Purification

Protein A affinity chromatography

Pureté

> 95 % (by SDS-PAGE)

Immunogène

Recombinant cytoplasmic domain (aa 91-244) of human NTAL

Isotype

IgG1
anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (AA 91-243), (Cytoplasmic Domain) antibody
LAT2 Reactivité: Humain, Souris WB, IP, ICC, ICFC Hôte: Souris Monoclonal NAP-07 unconjugated

anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (AA 30-209) antibody
LAT2 Reactivité: Humain WB, IHC, IP, ICC Hôte: Lapin Polyclonal unconjugated

anti-Linker For Activation of T Cells Family, Member 2 (LAT2) antibody
LAT2 Reactivité: Souris, Rat IHC, IF Hôte: Lapin Polyclonal unconjugated

anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (AA 91-243), (Cytoplasmic Domain) antibody (PE)
LAT2 Reactivité: Humain, Souris ICFC Hôte: Souris Monoclonal NAP-07 PE

anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (AA 32-192) antibody
LAT2 Reactivité: Rat WB Hôte: Lapin Polyclonal unconjugated

anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (AA 124-203), (Cytoplasmic Domain), (N-Term) antibody
LAT2 Reactivité: Souris WB Hôte: Lapin Polyclonal unconjugated

anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (AA 196-212) antibody
LAT2 Reactivité: Humain WB, IHC, IP, IHC (p), Inhibition Hôte: Souris Monoclonal NAP-03 unconjugated

anti-Linker For Activation of T Cells Family, Member 2 (LAT2) antibody
LAT2 Reactivité: Humain WB, FACS Hôte: Souris Monoclonal 24GB15935 unconjugated

anti-Linker For Activation of T Cells Family, Member 2 (LAT2) antibody
LAT2 Reactivité: Humain WB, FACS Hôte: Souris Monoclonal 24GB14270 unconjugated

anti-Linker For Activation of T Cells Family, Member 2 (LAT2) antibody
LAT2 Reactivité: Humain WB, FACS Hôte: Souris Monoclonal 24GB14275 unconjugated

Indications d'application

Immunoprecipitation. Western Blot: Recommended dilution: 2 mg/mL, incubation 60 min Positive control: RAMOS human lymphoma cell line Negative control: HeLa human cervix carcinoma cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non- reducing SDS-PAGE sample buffer. Application note: Non-Reducing conditions. 12 % separating SDS-PAGE gel. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Concentration

1.0 mg/mL

Buffer

Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

4 °C/-20 °C

Stockage commentaire

Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Date de péremption

12 months
Nørsett, Laegreid, Langaas, Wörlund, Fossmark, Waldum, Sandvik: "Molecular characterization of rat gastric mucosal response to potent acid inhibition." dans: Physiological genomics, Vol. 22, Issue 1, pp. 24-32, (2007) (PubMed).

Tkaczyk, Horejsi, Iwaki, Draber, Samelson, Satterthwaite, Nahm, Metcalfe, Gilfillan: "NTAL phosphorylation is a pivotal link between the signaling cascades leading to human mast cell degranulation following Kit activation and Fc epsilon RI aggregation." dans: Blood, Vol. 104, Issue 1, pp. 207-14, (2004) (PubMed).

Volná, Lebduska, Dráberová, Símová, Heneberg, Boubelík, Bugajev, Malissen, Wilson, Horejsí, Malissen, Dráber: "Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL." dans: The Journal of experimental medicine, Vol. 200, Issue 8, pp. 1001-13, (2004) (PubMed).
Antigène

LAT2 (Linker For Activation of T Cells Family, Member 2 (LAT2))

Autre désignation

LAT2 / WBSCR15

Sujet

NTAL (Non-T cell activation linker), also known as LAB (Linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.Synonyms: HSPC046, LAB, LLinker for activation of B-cells, Linker for activation of T-cells family member 2, Membrane-associated adapter molecule, NTAL, Non-T-cell activation linker, WBS15, WBSCR5, Williams-Beuren syndrome chromosomal region 15 protein

ID gène

7462

UniProt

Q9GZY6

Pathways

Fc-epsilon Receptor Signaling Pathway, BCR Signaling