L’anticorps anti-SERPINA1 Polyclonal Lapin est utilisé pour la détection de SERPINA1 dans des échantillons de Humain. Il a été validé pour IHC et IHC (p).
IHC, ICC (frozen or formalin–fixed paraffin-embedded (FFPE) tissue sections, cell smears) 1:50-1:200 using streptavidin-Biotin system or polymer system. Prediluted antibody is used directly without dilution. FFPE tissue sections require antigen retriever (boiling tissue in 10 mM citrate pH 6.0 for 10-20 minutes, followed by cooling at RT for 15-20 minutes). The optimum dilution should be determined by the individual lab. Positive Control: Human tonsil, liver. Cellular Localization: Cytoplasmic staining of macrophages.
Restrictions
For Research Use only
Format
Liquid
Buffer
The antibody is supplied with 1% BSA in PBS with 0.05% azide, pH 7.4. The Prediluted antibody is supplied in our Universal antibody dilution buffer (AR-6526) green in color.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C
Antigène
SERPINA1
(serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1))
Autre désignation
a-1 Antitrypsin
Sujet
Alpha-1-Antitrypsin (AAT) is a 51 kDa glycoprotein synthesized in the liver. It is a serum protease inhibitor in neutrophil elastase, collagenase and chymotrypsin. This protein is a serum trypsin inhibitor, the correct name is alpha-1-proteinase inhibitor (A1PI). Disorders of this enzyme include, deficiency, in a hereditary disorder, this causes degradation of lung tissue and causes pulmonary emphysema, it also causes chronic liver disease and hepatocarcinoma. AAT is present in normal liver cells, histocytes, monocytes and a variety of epithelial and mesenchymal tumors. Increase in serum levels of AAT is also reported in rheumatoid arthritis, bacterial infection, vasculistis and carcinomatosis. Intended USE: Immunohistochemistry (IHC) and Immunocytchemistry (ICC)