Ezrin anticorps (pThr567)
Aperçu rapide pour Ezrin anticorps (pThr567) (ABIN362229)
Antigène
Voir toutes Ezrin (EZR) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- pThr567
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Specificité
- The antibody detects endogenous level of Ezrin only when phosphorylated at threonine 567.
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Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
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Immunogène
- Peptide sequence around phosphorylation site of pThr567 (Y-K-T (p) -L-R) derived from Human Ezrin. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
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Isotype
- IgG
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Indications d'application
- Western blotting: 1:500-1:1000
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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: "Expression of Ezrin and phosphorylated Ezrin (pEzrin) in pancreatic ductal adenocarcinoma." dans: Cancer investigation, Vol. 28, Issue 3, pp. 242-7, (2010) (PubMed).
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- Ezrin (EZR)
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Autre désignation
- Ezrin
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Sujet
- Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
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Poids moléculaire
- 81 kDa
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NCBI Accession
- NP_001104
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UniProt
- P15311
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Pathways
- Maintenance of Protein Location
Antigène
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