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MDM2 anticorps (pSer166)

Cité dans 2 publications. Cet anticorps anti-MDM2 Polyclonal Lapin (ABIN362481) détecte spécifiquement MDM2 dans WB, IHC et IF. L’anticorps est réactif avec des échantillons de Humain.

(2 références)

N° du produit ABIN362481

268,40 €

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Destination: France

Envoi sous 10 à 14 jours ouvrables

Aperçu rapide pour MDM2 anticorps (pSer166) (ABIN362481)

Antigène

Voir toutes MDM2 Anticorps

MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Reactivité

Humain

Hôte

Lapin

Clonalité

Polyclonal

Conjugué

Cet anticorp MDM2 est non-conjugé

Application

Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Discover our top product MDM2 Anticorps primaire

Épitope
27

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pSer166

Specificité

The antibody detects endogenous level of MDM2 only when phosphorylated at serine 166.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogène

Peptide sequence around phosphorylation site of pSer166 (A-I-S (p) -E-T) derived from Human MDM2. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype

IgG
anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 101-200) antibody
MDM2 Reactivité: Humain WB, ELISA, IHC (p), PLA, RNAi Hôte: Souris Monoclonal 1A7 unconjugated

anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Center) antibody
MDM2 Reactivité: Humain, Souris WB, IF, IHC (p), IP, ICC, ChIP Hôte: Lapin Polyclonal unconjugated

anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 130-230) antibody
MDM2 Reactivité: Humain WB, IHC, IF Hôte: Lapin Polyclonal unconjugated

anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 141-176) antibody
MDM2 Reactivité: Humain WB, IF, IHC (p), FACS Hôte: Lapin Polyclonal RB7981 unconjugated

anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (pSer166) antibody
MDM2 Reactivité: Humain, Souris, Singe WB, IHC, ELISA, IF, ICC Hôte: Lapin Polyclonal unconjugated

anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 26-169) antibody
MDM2 Reactivité: Humain IHC, ELISA, FACS Hôte: Souris Monoclonal 3C1E3 unconjugated

anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 393-424) antibody
MDM2 Reactivité: Humain, Souris WB, IHC, ELISA Hôte: Lapin Polyclonal unconjugated

anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (pSer186) antibody
MDM2 Reactivité: Humain WB, ELISA, IHC (p), ICC, IF (cc), IF (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 200-430) antibody
MDM2 Reactivité: Humain WB, IF Hôte: Lapin Polyclonal unconjugated

anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 119-438) antibody
MDM2 Reactivité: Humain WB, IHC, IHC (p) Hôte: Souris Monoclonal 1E6 unconjugated

Indications d'application

Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

4 °C/-20 °C

Stockage commentaire

Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Lei, Velez, Kazlauskas: "Pathological signaling via platelet-derived growth factor receptor {alpha} involves chronic activation of Akt and suppression of p53." dans: Molecular and cellular biology, Vol. 31, Issue 9, pp. 1788-99, (2011) (PubMed).

Lei, Velez, Cui, Samad, Maberley, Matsubara, Kazlauskas: "N-acetylcysteine suppresses retinal detachment in an experimental model of proliferative vitreoretinopathy." dans: The American journal of pathology, Vol. 177, Issue 1, pp. 132-40, (2010) (PubMed).
Antigène

MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Autre désignation

MDM2

Sujet

This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues

Poids moléculaire

90 kDa

NCBI Accession

NP_002383

UniProt

Q00987

Pathways

Signalisation p53, Signalisation PI3K-Akt, Cycle Cellulaire, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Autophagy, Ubiquitin Proteasome Pathway