Lapin anti-Souris IgG (F(ab')2 Region) Anticorps - Preadsorbed
Aperçu rapide pour Lapin anti-Souris IgG (F(ab')2 Region) Anticorps - Preadsorbed (ABIN101844)
Antigène
Clonalité
Application
Isotype
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Épitope
- F(ab')2 Region
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Reactivité
- Souris
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Hôte
- Lapin
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N° du produit (Fournisseur)
- 610-4121
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Fournisseur
- Rockland
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Fonction
- Mouse IgG F(ab')2 Antibody Pre-Adsorbed
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Réactivité croisée (Details)
- Minimal crossreactivity against Human Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG, Mouse IgG F(ab')2 and Mouse Serum. No reaction was observed against Mouse IgG F(c) or Human Serum Proteins.
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Attributs du produit
- Anti-Mouse IgG antibody generated in rabbit detects specifically mouse IgG (H&L).
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Purification
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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Stérilité
- Sterile filtered
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Immunogène
- Optional[Immunogen]: Mouse IgG F(ab')2 fragment
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Goat anti-Mouse IgG antibody (DyLight 800)
WB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Mouse IgG antibody (DyLight 800) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Mouse IgG antibody (DyLight 488) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 488
Rabbit anti-Mouse IgG (Heavy & Light Chain) antibodyELISA, IHC, WB Polyclonal IgG unconjugated Fab fragment
Goat anti-Mouse IgG antibody (DyLight 680)WB, FLISA, FM, DB Polyclonal IgG DyLight 680
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (Cy5)IHC, FACS Polyclonal IgG Cy5
Rabbit anti-Mouse IgG antibody (HRP) - PreadsorbedELISA, WB, IHC (fro), IHC (p), IHC (rs) Polyclonal IgG HRP F(ab')2 fragment
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (PE) - PreadsorbedELISA, FACS Polyclonal IgG PE F(ab')2 fragment
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (DyLight 488)IHC, FACS, IF, ICC Polyclonal IgG DyLight 488
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Indications d'application
- Application Note: Anti-Mouse IgG F(ab')2 antibody has been tested by ELISA and is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 2.193 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
, Preservative:0.01 % (w/v) Sodium Azide -
Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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Date de péremption
- 12 months
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- IgG
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Classe de substances
- Antibody
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Sujet
- Anti-Mouse IgG F(ab')2 Antibody generated in rabbit recognizes the dimeric Fab portion of the mouse IgG molecule. Mouse IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind mouse IgG F(c) will not bind mouse IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Antigène
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