Lapin anti-Souris IgA, IgG, IgM Anticorps (Biotin) - Preadsorbed
ELISA, IHC, ICC
Polyclonal
IgG
Biotin
N° du produit ABIN458462
705,22 €
Plus frais de livraison 40,00 € et TVA
1 mL
Destination:
France
Envoi sous 2 à 4 jours ouvrables
Aperçu rapide pour Lapin anti-Souris IgA, IgG, IgM Anticorps (Biotin) - Preadsorbed (ABIN458462)
Antigène
IgA, IgG, IgM
Clonalité
Polyclonal
Conjugué
Biotin
Application
ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Isotype
IgG
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Reactivité
- Souris
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Hôte
- Lapin
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Specificité
- The reactivity of the antiserum is directed to the major isotypes of the mouse immunoglobulin system (classes and both light chain types) including antibodies to common determinants, to class and to the surface determinants of the common Fab portion, as tested by immunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulins. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal mouse plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained.
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Attributs du produit
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Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to mouse IgG, IgA and IgM, heavy and light chains, adsorbed for human cross-reactivity.
Biotin/IgG protein molar ratio (B/P) is approximately 3.9. -
Purification
- Preadsorption: Immunoaffinity adsorbed using insolubilized Ig-depleted mouse.
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Immunogène
- Purified polyclonal mouse IgG, and homogenous IgA and IgM isolated from mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
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Indications d'application
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In immunocytochemical and immunohistochemical staining of monoclonal mouse monoclonal antibodies in the presence of human cell or serum components at the cellular and subcellular level. As a second step an avidin or streptavidin conjugate of the user's choice has to be used. This immunoconjugate is not pre-diluted.
The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:100 and 1:500, in ELISA and comparable non-precipitating antibody-binding assays between 1:1,000 and 1:10,000. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- It is reconstituted by adding 1 mL sterile distilled water. Spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.
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Concentration
- 10 mg/mL
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Buffer
- Biotin-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
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Agent conservateur
- Without preservative
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Conseil sur la manipulation
- Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and prefe rably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
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- IgA, IgG, IgM
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Autre désignation
- IgG + IgA + IgM
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Classe de substances
- Antibody
Antigène
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