ACP1 anticorps (N-Term)
Aperçu rapide pour ACP1 anticorps (N-Term) (ABIN306667)
Antigène
Voir toutes ACP1 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- N-Term
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Fonction
- Sheep anti Human PTP (HCPTP)
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Attributs du produit
- Low molecular weight protein tyrosine phosphtases, including the human red cell phosphatase, HCPTP, are widely expressed cytosolic proteins of approximately 18 kDa that exist in distinct isoforms and are highly selective for phosphotyrosine over phosphoserine or phosphotyrosine. The HCPTP-A and HCPTP-B proteins expressed in human red cells and placenta are fast and slow forms of red cell acid phosphatase5. Like other tyrosine phosphatases, HCPTP is sensitive to inhibition by vanadate, and has a catalytic mechanism that involves formation of a cystinyl-phosphate intermediate6. The crystal structure of the bovine heart derived enzyme BHPTP shows a four-stranded central parallel b sheet with flanking a helices and an active site cysteine residue in the typical tyrosine phosphatase sequence context, CXXXXXR. Biological actions and cellular substrates have not been fully elucidated. It is expressed in a wide range of cell types, and structural homologues are expressed in yeast. A catalytically inactive LMW-PTP functions to promote cell division and binds to tyrosine-phosphorylated PDGF receptors1. The HCPTPA isoform interacts with receptor tyrosine kinases, EphB1 and VEGFR2 (flk-1)2,4. Its overexpression inhibits VEGF-induced endothelial proliferation and migration, and its recruitment to EphB1 complexes is crucial to downstream signaling between EphB1 and integrins that mediate cell-matrix attachment3.
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Purification
- Ammonium Sulfate Precipitation
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Immunogène
- The human protein, HCPTPA (GB Accession #M83653) was subcloned into pRSET to express a His6 N terminal fusion protein. This protein was expressed in E. coli and purified to homogeneity on Ni affinity matrix.
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Isotype
- IgG
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anti-Acid Phosphatase 1, Soluble (ACP1) (AA 1-158) antibody
ACP1 Reactivité: Humain WB, ELISA Hôte: Souris Monoclonal 4B10 unconjugated
anti-Acid Phosphatase 1, Soluble (ACP1) (AA 1-158) antibodyACP1 Reactivité: Humain WB, ELISA Hôte: Souris Monoclonal 2A3 unconjugated
anti-Acid Phosphatase 1, Soluble (ACP1) (AA 33-61), (N-Term) antibodyACP1 Reactivité: Humain WB, IF, FACS, IHC (p) Hôte: Lapin Polyclonal RB23949 unconjugated
anti-Acid Phosphatase 1, Soluble (ACP1) (AA 15-144) antibodyACP1 Reactivité: Humain WB Hôte: Lapin Polyclonal unconjugated
anti-Acid Phosphatase 1, Soluble (ACP1) (AA 1-158) antibodyACP1 Reactivité: Rat WB, IHC, ICC, IF Hôte: Lapin Polyclonal unconjugated
anti-Acid Phosphatase 1, Soluble (ACP1) (AA 1-158) antibodyACP1 Reactivité: Humain WB, IHC, ELISA Hôte: Lapin Polyclonal unconjugated
anti-Acid Phosphatase 1, Soluble (ACP1) antibodyACP1 Reactivité: Humain, Rat, Souris, Boeuf (Vache) WB, IHC, IP, IHC (p) Hôte: Mouton Polyclonal unconjugated
anti-Acid Phosphatase 1, Soluble (ACP1) (AA 21-70) antibodyACP1 Reactivité: Humain, Boeuf (Vache), Lapin, Cheval, Singe, Poulet, Drosophila melanogaster WB, IHC, IHC (p) Hôte: Lapin Polyclonal unconjugated
anti-Acid Phosphatase 1, Soluble (ACP1) (AA 1-158) antibodyACP1 Reactivité: Humain WB, ICC, ELISA, IF Hôte: Souris Monoclonal AT5C11 unconjugated
anti-Acid Phosphatase 1, Soluble (ACP1) (AA 40-69), (N-Term) antibodyACP1 Reactivité: Humain WB, FACS, IHC (p), EIA Hôte: Lapin Polyclonal unconjugated
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Indications d'application
- Antbibody can be used for Western blotting and immunoprecipitation (1-10 μg/mL. Optimal concentration should be evaluated by serial dilutions.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/ml
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Buffer
- phosphate buffered saline with 0.08 % sodium azide
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- -20 °C
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Stockage commentaire
- Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
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