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HA-Tag anticorps

WB, ELISA, IP Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN100176
  • Antigène Tous les produits HA-Tag
    HA-Tag
    Reactivité
    Veuillez nous consulter SVP
    Hôte
    • 74
    • 73
    • 13
    • 7
    • 1
    • 1
    Lapin
    Clonalité
    • 84
    • 82
    Polyclonal
    Conjugué
    • 69
    • 15
    • 13
    • 11
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp HA-Tag est non-conjugé
    Application
    • 133
    • 61
    • 38
    • 28
    • 19
    • 16
    • 14
    • 14
    • 9
    • 6
    • 6
    • 6
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunoprecipitation (IP)
    Séquence
    YPYDVPDYA
    Specificité
    This affinity purified Anti-HA antibody is directed against the HA motif and is useful in determining its presence in various assays. This polyclonal anti-HA tag antibody detects over-expressed proteins containing the HA epitope tag. To date, this antibody has reacted with all HA-tagged proteins tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.
    Attributs du produit
    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. Most often, sequences encoding the epitope tag are included with the target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows Anti epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of
    Stérilité
    Sterile filtered
    Immunogène
    This antibody was purified from whole rabbit serum prepared by repeated immunizations with the 9-aa epitope tag peptide YPYDVPDYA (114-122) from hemagglutinin influenza conjugated to KLH using maleimide. A residue of cysteine was added to the carboxy terminal end to facilitate coupling.
    Immunogen Type: Peptide
    Isotype
    IgG
  • Indications d'application
    Anti-HA is optimally suited for monitoring the expression of HA-tagged fusion proteins. As such, anti-HA/HA can be used to identify fusion proteins containing the HA epitope. The antibody recognizes the HA epitope tag fused to the amino- or carboxy- termini of targeted proteins, as expressed in many commonly used expression vectors. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and HA containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation, immunocytochemistry, and other immunodetection techniques. Affinity purification of the polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.95 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
    Date de péremption
    12 months
  • Wallis, Ventimiglia, Otigbah, Infante, Cuesta-Geijo, Kidiyoor, Carbajal, Fleck, Foiani, Garcia-Manyes, Martin-Serrano, Agromayor: "The ESCRT machinery counteracts Nesprin-2G-mediated mechanical forces during nuclear envelope repair." dans: Developmental cell, Vol. 56, Issue 23, pp. 3192-3202.e8, (2021) (PubMed).

    Merigliano, Burla, La Torre, Del Giudice, Teo, Liew, Chojnowski, Goh, Olmos, Maccaroni, Giubettini, Chiolo, Carlton, Raimondo, Vernì, Stewart, Rhodes, Wright, Burke, Saggio: "AKTIP interacts with ESCRT I and is needed for the recruitment of ESCRT III subunits to the midbody." dans: PLoS genetics, Vol. 17, Issue 8, pp. e1009757, (2021) (PubMed).

    Alvarez-Castelao, Tom Dieck, Fusco, Donlin-Asp, Perez, Schuman: "The switch-like expression of heme-regulated kinase 1 mediates neuronal proteostasis following proteasome inhibition." dans: eLife, Vol. 9, (2020) (PubMed).

    Nuwer, Fleck: "Anterograde trafficking signals in GABAA subunits are required for functional expression." dans: Channels (Austin, Tex.), Vol. 13, Issue 1, pp. 440-454, (2020) (PubMed).

    Rohde, Becker, Krähling: "Marburg virus regulates the IRE1/XBP1-dependent unfolded protein response to ensure efficient viral replication." dans: Emerging microbes & infections, Vol. 8, Issue 1, pp. 1300-1313, (2020) (PubMed).

    Stedden, Menegas, Zajac, Williams, Cheng, Özkan, Horne-Badovinac: "Planar-Polarized Semaphorin-5c and Plexin A Promote the Collective Migration of Epithelial Cells in Drosophila." dans: Current biology : CB, Vol. 29, Issue 6, pp. 908-920.e6, (2020) (PubMed).

    Pati, Salvi, Kallianpur, Vaidya, Banerjee, Maiti, Clement, Vaidya: "Chemogenetic Activation of Excitatory Neurons Alters Hippocampal Neurotransmission in a Dose-Dependent Manner." dans: eNeuro, Vol. 6, Issue 6, (2020) (PubMed).

    Cousineau, Lescouzères, Taupignon, Delgado-Zabalza, Valjent, Baufreton, Le Bon-Jégo: "Dopamine D2-Like Receptors Modulate Intrinsic Properties and Synaptic Transmission of Parvalbumin Interneurons in the Mouse Primary Motor Cortex." dans: eNeuro, Vol. 7, Issue 3, (2020) (PubMed).

    Ventimiglia, Cuesta-Geijo, Martinelli, Caballe, Macheboeuf, Miguet, Parnham, Olmos, Carlton, Weissenhorn, Martin-Serrano: "CC2D1B Coordinates ESCRT-III Activity during the Mitotic Reformation of the Nuclear Envelope." dans: Developmental cell, Vol. 47, Issue 5, pp. 547-563.e6, (2019) (PubMed).

    Yamada, Murata, Adachi, Itoh, Kameoka, Igarashi, Kato, Araki, Huganir, Dawson, Yanagawa, Okamoto, Iijima, Sesaki: "Mitochondrial Stasis Reveals p62-Mediated Ubiquitination in Parkin-Independent Mitophagy and Mitigates Nonalcoholic Fatty Liver Disease." dans: Cell metabolism, Vol. 28, Issue 4, pp. 588-604.e5, (2019) (PubMed).

    Milewska, Kindler, Vkovski, Zeglen, Ochman, Thiel, Rajfur, Pyrc: "APOBEC3-mediated restriction of RNA virus replication." dans: Scientific reports, Vol. 8, Issue 1, pp. 5960, (2019) (PubMed).

    Kijima, Prince, Tigue, Yim, Schwartz, Beebe, Lee, Budzynski, Williams, Trepel, Sistonen, Calderwood, Neckers: "HSP90 inhibitors disrupt a transient HSP90-HSF1 interaction and identify a noncanonical model of HSP90-mediated HSF1 regulation." dans: Scientific reports, Vol. 8, Issue 1, pp. 6976, (2018) (PubMed).

    Ulu, Oh, Zuo, Frost: "Stress-activated MAPKs and CRM1 regulate the subcellular localization of Net1A to control cell motility and invasion." dans: Journal of cell science, Vol. 131, Issue 3, (2018) (PubMed).

    Bohn, Thummar, York, Raymond, Brown, Bieniasz, Hatziioannou, Smith: "APOBEC3H structure reveals an unusual mechanism of interaction with duplex RNA." dans: Nature communications, Vol. 8, Issue 1, pp. 1021, (2018) (PubMed).

    Yue, Nabar, Shi, Kamenyeva, Xiao, Hwang, Wang, Kehrl: "SARS-Coronavirus Open Reading Frame-3a drives multimodal necrotic cell death." dans: Cell death & disease, Vol. 9, Issue 9, pp. 904, (2018) (PubMed).

    Kim, Wang, Sanchez, Coukos, von Zastrow, Ting: "Time-gated detection of protein-protein interactions with transcriptional readout." dans: eLife, Vol. 6, (2018) (PubMed).

    Kanter, Lykken, Avesar, Weible, Dickinson, Dunn, Borgesius, Roudi, Kentros: "A Novel Mechanism for the Grid-to-Place Cell Transformation Revealed by Transgenic Depolarization of Medial Entorhinal Cortex Layer II." dans: Neuron, Vol. 93, Issue 6, pp. 1480-1492.e6, (2017) (PubMed).

    York, Kutluay, Errando, Bieniasz: "The RNA Binding Specificity of Human APOBEC3 Proteins Resembles That of HIV-1 Nucleocapsid." dans: PLoS pathogens, Vol. 12, Issue 8, pp. e1005833, (2017) (PubMed).

    Blanco-Melo, Gifford, Bieniasz: "Co-option of an endogenous retrovirus envelope for host defense in hominid ancestors." dans: eLife, Vol. 6, (2017) (PubMed).

    Meese, Cepeda, Gahlen, Adams, Ficner, Ricci, Heller, Reisinger, Herget: "Activity-Dependent Phosphorylation by CaMKIIδ Alters the Ca2+ Affinity of the Multi-C2-Domain Protein Otoferlin." dans: Frontiers in synaptic neuroscience, Vol. 9, pp. 13, (2017) (PubMed).

  • Antigène
    HA-Tag
    Autre désignation
    HA tag (HA-Tag Produits)
    Classe de substances
    Tag
    Sujet
    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. Most often, sequences encoding the epitope tag are included with the target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows Anti epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of
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