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Chèvre anti-Âne IgG (Heavy & Light Chain) Anticorps

ELISA, IHC, WB Polyclonal IgG unconjugated
N° du produit ABIN101122
  • Antigène Tous les produits IgG
    IgG
    Épitope
    • 2332
    • 499
    • 411
    • 188
    • 129
    • 122
    • 92
    • 29
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    • 15
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    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Heavy & Light Chain
    Reactivité
    • 695
    • 672
    • 561
    • 458
    • 366
    • 239
    • 198
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    • 131
    • 122
    • 98
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    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Âne
    Hôte
    • 1729
    • 1474
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    • 206
    • 118
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    • 33
    • 31
    • 21
    • 17
    • 15
    • 11
    • 11
    • 1
    Chèvre
    Clonalité
    • 3360
    • 167
    • 1
    Polyclonal
    Application
    • 1839
    • 1800
    • 1791
    • 1500
    • 962
    • 934
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    • 1
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    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Specificité
    IgG (H&L)
     Réactivité croisée
    Âne
    Attributs du produit
    Concentration Definition: by UV absorbance at 280 nm
    Purification
    Anti-DONKEY IgG (H&L) (GOAT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Donkey IgG and Donkey Serum.
    Immunogène

    Immunogen: Donkey IgG whole molecule

    Isotype
    IgG
  • Indications d'application

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: Anti-DONKEY IgG (H&L) (GOAT) Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Specific conditions should be optimized by user.

    ELISA Dilution: 1:20,000 - 1:100,000

    Western Blot Dilution: 1:2,000 - 1:10,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 5.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    10.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    RT,4 °C,-20 °C
    Date de péremption
    12 months
  • Antigène
    IgG
    Abstract
    IgG Produits
    Classe de substances
    Antibody
    Sujet

    Synonyms: goat anti-donkey IgG, immunoglobulin, donkey antibodies, goat antibody

    Background: Anti-Donkey IgG Antibody generated in goat detects donkey IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Anti-Donkey IgG antibody is ideal for investigators involved in serum rotein component research.

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