Lapin anti-Souris IgG (Fc Region) Anticorps - Preadsorbed

N° du produit ABIN101867

Détail du produit

Antigène: IgG

Épitope: Fc Region

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificité: IgG F(c)

Réactivité croisée: Souris

Attributs du produit: Anti-Mouse IgG F(c) antibody generated in rabbit detects specifically mouse IgG. This secondary antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorption: immunoaffinity chromatography using Mouse IgG coupled to agarose beads

Stérilité: Sterile filtered

Immunogène:

Immunogen: Anti-Mouse IgG F(c) was produced by repeated immunization with mouse IgG F(c) fragment in rabbit.

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Mouse IgG F(c) Antibody is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.

ELISA Dilution: 1:100,000 - 1:250,000

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 2.1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Abstract: IgG Produits

Classe de substances: Antibody

Sujet:

Synonyms: rabbit anti-Mouse IgG F(c) Antibody, Rb-a-Mouse F(c), Mouse F(c) Antibody in rabbit, Mouse F(c) Secondary Antibody

Background: Anti-Mouse IgG F(c) generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of mouse IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.