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Lapin anti-Souris IgG2a (Heavy Chain) Anticorps (TRITC) - Preadsorbed

FACS, FLISA, FM Polyclonal IgG TRITC
N° du produit ABIN102382
  • Antigène Tous les produits IgG2a
    IgG2a
    Épitope
    • 42
    • 15
    • 7
    • 2
    • 2
    • 1
    • 1
    Heavy Chain
    Reactivité
    • 93
    • 18
    • 1
    Souris
    Hôte
    • 57
    • 22
    • 15
    • 13
    • 5
    Lapin
    Clonalité
    • 71
    • 28
    Polyclonal
    Conjugué
    • 20
    • 12
    • 11
    • 10
    • 8
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    TRITC
    Application
    • 50
    • 46
    • 35
    • 30
    • 23
    • 18
    • 10
    • 8
    • 7
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
    Specificité
    IgG2a
    Attributs du produit
    Concentration Definition: by UV absorbance at 280 nm
    Purification
    Preadsorption: Solid phase absorption
    Immunogène

    Immunogen: Mouse IgG2a heavy chain

    Isotype
    IgG
    Labeling Ratio
    2.4
  • Indications d'application

    Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    FLISA Dilution: 1:10,000 - 1:50,000

    Flow Cytometry Dilution: 1:500 - 1:2,500

    IF Microscopy Dilution: 1:1,000 - 1:5,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Product is photosensitive and should be protected from light.
    Stock
    RT,4 °C,-20 °C
    Date de péremption
    12 months
  • Antigène
    IgG2a
    Abstract
    IgG2a Produits
    Classe de substances
    Antibody
    Sujet

    Synonyms: Rabbit Anti-Mouse IgG2a (gamma 2a chain) Antibody rhodamine Conjugated, Rabbit Anti-Mouse IgG2a Antibody TRITC Conjugation

    Background: Anti-Mouse IgG2a Fluorescein Antibody generated in rabbit detects reactivity to Mouse IgG2a (Gamma 2a chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25 % of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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