PDCD4 anticorps (pSer457)

N° du produit ABIN1043728

Détail du produit anti-PDCD4 anticorps

Antigène: PDCD4 (Programmed Cell Death 4 (PDCD4))

Épitope: pSer457

Reactivité: Humain, Souris, Rat, Xenopus laevis

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PDCD4 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificité: Pdcd4 phospho S457 Antibody was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human Pdcd4 protein phosphorylated at Ser457.  A BLAST analysis was used to suggest cross-reactivity with Pdcd4 from human, mouse, rat and Xenopus based on 100% homology with the immunizing sequence.  Cross-reactivity with Pdcd4 from other sources has not been determined.

Attributs du produit: Programmed cell death 4 (Pdcd4) is a novel tumor supressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)-responsive promoters by c-Jun. Pdcd4 contains two Akt phosphoryl-ation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1-responsive promoters by c-Jun. Ideal for researchers involved in Cell Signaling, Cancer and Signal Transduction research.

Stérilité: Sterile filtered

Immunogène: Anti-Pdcd4 phospho S457 Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Ser457 of the human Pdcd4 protein.
Immunogen Type: Peptide

Clone: 2E10-C12

Isotype: IgG1 kappa

Information d'application

Indications d'application: This monoclonal antibody is suitable for ELISA, immunohistochemistry and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 62 kDa in size corresponding to phosphorylated Pdcd4 protein by western blotting in the appropriate cell lysate or extract.  This phospho-specific monoclonal antibody reacts with human Pdcd4 pS457 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Commentaires:

Gene Name: PDCD4

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Date de péremption: 12 months

Références pour anticorps anti-PDCD4 (ABIN1043728)

Huang, Wu, Loeb, Hsu, Heidersbach, Brincat, Horiuchi, Lebbink, Mo, Goga, McManus: "Up-regulation of miR-21 by HER2/neu signaling promotes cell invasion." dans: The Journal of biological chemistry, Vol. 284, Issue 27, pp. 18515-24, (2009) (PubMed).

Jansen, Camalier, Colburn: "Epidermal expression of the translation inhibitor programmed cell death 4 suppresses tumorigenesis." dans: Cancer research, Vol. 65, Issue 14, pp. 6034-41, (2005) (PubMed).

Détails sur PDCD4

Antigène: PDCD4 (Programmed Cell Death 4 (PDCD4))

Autre désignation: Pdcd4 (PDCD4 Produits)

Synonymes: anticorps H731, anticorps D19Ucla1, anticorps Ma3, anticorps Tis, anticorps Dug, anticorps cb45, anticorps pdcd4, anticorps sb:cb45, anticorps wu:fc84b08, anticorps h731, anticorps MGC69337, anticorps PDCD4, anticorps wu:fa55g03, anticorps wu:fb08a11, anticorps wu:fe01a02, anticorps zgc:66311, anticorps programmed cell death 4, anticorps programmed cell death 4b, anticorps programmed cell death 4 L homeolog, anticorps programmed cell death 4 (neoplastic transformation inhibitor), anticorps programmed cell death 4a, anticorps PDCD4, anticorps Pdcd4, anticorps pdcd4b, anticorps pdcd4.L, anticorps pdcd4, anticorps pdcd4a

Sujet: Programmed cell death 4 (Pdcd4) is a novel tumor supressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)-responsive promoters by c-Jun. Pdcd4 contains two Akt phosphoryl-ation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1-responsive promoters by c-Jun. Ideal for researchers involved in Cell Signaling, Cancer and Signal Transduction research.
Synonyms: Death up-regulated gene protein antibody, Dug antibody, H731 antibody, Ma3 antibody, Neoplastic transformation inhibitor antibody, Neoplastic transformation inhibitor protein antibody, Nuclear antigen H731 antibody

ID gène: 27250, 21735596

UniProt: Q53EL6