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GDF15 anticorps (Biotin)

GDF15 Reactivité: Humain, Souris WB, ELISA Hôte: Lapin Polyclonal Biotin
N° du produit ABIN1043807
  • Antigène Voir toutes GDF15 Anticorps
    GDF15 (Growth Differentiation Factor 15 (GDF15))
    Reactivité
    • 89
    • 30
    • 23
    • 1
    • 1
    Humain, Souris
    Hôte
    • 86
    • 12
    • 3
    • 1
    Lapin
    Clonalité
    • 86
    • 15
    • 1
    Polyclonal
    Conjugué
    • 53
    • 15
    • 9
    • 8
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp GDF15 est conjugé à/à la Biotin
    Application
    • 87
    • 54
    • 36
    • 16
    • 13
    • 13
    • 9
    • 7
    • 6
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA
    Specificité
    This antibody reacts with endogenous NAG-1 protein from human tissues.  A BLAST analysis using the immunizing recombinant protein suggests reactivity with NAG-1 from human and chimpanzee based on 100% and 98% homology, respectively, and with macaque based on an 94% homology, and with mouse and rat based on 70% homology with the immunizing sequence. Cross-reactivity with NAG-1 from other sources has not been determined.
    Purification
    purified
    Immunogène
    This Protein-A purified antibody was prepared by repeated immunizations with an MBP-tagged recombinant protein produced in E.coli corresponding to mature human NAG-1 protein.  Cross reactivity to MBP was removed via cross-adsorption chromatography.
    Immunogen Type: RecombinantProtein
    Isotype
    IgG
    Top Product
    Discover our top product GDF15 Anticorps primaire
  • Indications d'application
    This Protein-A purified, MBP-cross-adsorbed antibody is suitable for ELISA and western blotting of human and mouse NAG-1 protein.   For detection of NAG-1 in human serum, a sandwich ELISA is suggested using this antibody in combination with anti-NAG-1/GDF15 C-terminal specific antibodies.  This antibody is useful in dual antibody immunometric assays (EIA). Specific conditions for reactivity should be optimized by the end user.  Expect bands in western blots of approximately 14 kDa in size corresponding to NAG-1 monomer using the appropriate cell lysate or extract.
    Commentaires

    Gene Name: GDF15

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
    Date de péremption
    12 months
  • Baek, Eling: "Changes in gene expression contribute to cancer prevention by COX inhibitors." dans: Progress in lipid research, Vol. 45, Issue 1, pp. 1-16, (2006) (PubMed).

  • Antigène
    GDF15 (Growth Differentiation Factor 15 (GDF15))
    Autre désignation
    NAG-1 (GDF15 Produits)
    Synonymes
    anticorps GDF15, anticorps GDF-15, anticorps MIC-1, anticorps MIC1, anticorps NAG-1, anticorps PDF, anticorps PLAB, anticorps PTGFB, anticorps SBF, anticorps growth differentiation factor 15, anticorps GDF15, anticorps Gdf15
    Sujet
    Non-steroidal anti-inflammatory drug (NSAID) activated gene (NAG-1) is a member of the transforming growth factor-beta (TGF-beta) superfamily. NAG-1 is also known as Macrophage Inhibitory Cytokine-1 (MIC-1), Growth Differentiation Factor 15 (GDF15), Placental Bone Morphogenetic Protein (PLAB), or Prostate Derived Factor (PDF). NAG-1 is expressed in human placenta, prostate and colon. It possesses antitumorigenic and proapoptotic activities. NAG-1 expression is dramatically increased in inflammation, injury and malignancy. Increase of NAG-1 expression is a feature of many cancers including breast, colon, pancreas and prostate. In a number of studies, NAG-1 expression was increased by a number of NSAIDs. This increase in expression may correlate with the chemopreventive effect NSAIDs seem to have with certain cancers. NAG-1 expression is also induced by PPAR gamma ligands and by several dietary compounds such as conjugated linoleic acids (CLAs), naturally occurring fatty acids in ruminant food products, indoles, epicatechin gallate, and genistein. Induced expression of NAG-1 results in stimulation of apoptosis and inhibition of cell growth. Inhibition of NAG-1 induced expression by small interference RNA (siRNA) results in repression of induced apoptosis. NAG-1 expression is regulated by a numbers of transcription factors such as ERG-1 and Sp1. EGR-1 may be necessary for NSAID-induced NAG-1 expression. The study of expression of NAG-1 proteins, including variants, is important to define their potential role as serum biomarkers for cancer diagnosis, treatment monitoring, epidemiology study, and nutrition surveys.
    Synonyms: NAG-1, GDF15, MIC-1, nonsteroidal anti-inflammatory drug-activated gene, NSAID-activated gene 1 protein, growth differentiation factor 15, macrophage inhibitory compound 1, prostate-derived factor
    ID gène
    9518
    UniProt
    Q99988
    Pathways
    SARS-CoV-2 Protein Interactome
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