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ADRA1D anticorps (Extracellular Domain)

ADRA1D Reactivité: Humain, Cheval, Chien, Singe, Porc, Cobaye IHC, IHC (p) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN1048312
  • Antigène Voir toutes ADRA1D Anticorps
    ADRA1D (Adrenergic Receptor, alpha 1d (ADRA1D))
    Épitope
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Extracellular Domain
    Reactivité
    • 23
    • 15
    • 12
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    Humain, Cheval, Chien, Singe, Porc, Cobaye
    Hôte
    • 23
    Lapin
    Clonalité
    • 23
    Polyclonal
    Conjugué
    • 16
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp ADRA1D est non-conjugé
    Application
    • 9
    • 8
    • 7
    • 4
    • 4
    • 4
    • 1
    • 1
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marque
    IHC-plus™
    Specificité
    Human ADRA1D. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except ADRA1B (56 % ).
    Homologie
    Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Dog, Panda, Horse, Pig, Guinea pig (100%) Mouse, Rat, Bovine, Hamster, Rabbit, Opossum (94%) Bat (89%).
    Purification
    Immunoaffinity purified
    Immunogène
    Synthetic 18 amino acid peptide from 2nd extracellular domain of human ADRA1D. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Dog, Panda, Horse, Pig, Guinea pig (100%), Mouse, Rat, Bovine, Hamster, Rabbit, Opossum (94%), Bat (89%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product ADRA1D Anticorps primaire
  • Indications d'application
    Approved: IHC, IHC-P (2.5 μg/mL)
    Commentaires

    Target Species of Antibody: Human

    Procédure de l'essai

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Date de péremption
    12 months
  • Antigène
    ADRA1D (Adrenergic Receptor, alpha 1d (ADRA1D))
    Autre désignation
    ADRA1D (ADRA1D Produits)
    Synonymes
    anticorps Adra-1, anticorps Adra1, anticorps Adra1a, anticorps Gpcr8, anticorps Spr8, anticorps [a]1d, anticorps alpha1D-AR, anticorps Adrd1, anticorps ADRA1, anticorps ADRA1A, anticorps ADRA1R, anticorps ALPHA1, anticorps DAR, anticorps dJ779E11.2, anticorps adrenergic receptor, alpha 1d, anticorps adrenoceptor alpha 1D, anticorps Adra1d, anticorps ADRA1D
    Sujet
    Name/Gene ID: ADRA1D
    Subfamily: Adrenergic
    Family: GPCR

    Synonyms: ADRA1D, Alpha 1d, Alpha-1D adrenergic receptor, ADRA1R, Alpha-1D adrenoceptor, Alpha-1D adrenoreceptor, ALPHA1, Alpha-1D-adrenergic receptor, Alpha-adrenergic receptor 1a, Alpha1delta-AR, DAR, ADRA1, Adrenoceptor alpha 1D, Alpha-1A adrenergic receptor, DJ779E11.2
    ID gène
    146
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