Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

SMO anticorps (N-Term)

SMO Reactivité: Humain, Souris, Rat, Chien, Singe, Porc, Lapin, Boeuf (Vache), Hamster, Cheval, Roussette (Chauve-souris) WB, IHC, IHC (p), IF, ICC Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN1049353
  • Antigène Voir toutes SMO Anticorps
    SMO (Smoothened Homolog (Drosophila) (SMO))
    Épitope
    • 15
    • 8
    • 7
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivité
    • 52
    • 25
    • 22
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain, Souris, Rat, Chien, Singe, Porc, Lapin, Boeuf (Vache), Hamster, Cheval, Roussette (Chauve-souris)
    Hôte
    • 48
    • 4
    Lapin
    Clonalité
    • 49
    • 3
    Polyclonal
    Conjugué
    • 24
    • 5
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp SMO est non-conjugé
    Application
    • 44
    • 31
    • 13
    • 9
    • 6
    • 4
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)
    Marque
    IHC-plus™
    Specificité
    Human SMO. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Purification
    Immunoaffinity purified
    Immunogène
    Synthetic 15 amino acid peptide from N-terminal extracellular domain of human SMO. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Platypus (100%), Opossum (93%), Turkey, Chicken, Xenopus (87%), Zebrafish, Seq squirt (80%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product SMO Anticorps primaire
  • Indications d'application
    Approved: ICC, IF, IHC, IHC-P (10 μg/mL), WB

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.
    Commentaires

    Target Species of Antibody: Human

    Procédure de l'essai

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Date de péremption
    12 months
  • Antigène
    SMO (Smoothened Homolog (Drosophila) (SMO))
    Autre désignation
    SMO / Smoothened (SMO Produits)
    Synonymes
    anticorps FZD11, anticorps Gx, anticorps SMOH, anticorps Smoh, anticorps E130215L21Rik, anticorps bnb, anticorps smoothened, anticorps XSmo, anticorps smoothened, frizzled class receptor, anticorps smoothened, frizzled class receptor L homeolog, anticorps SMO, anticorps Smo, anticorps smo.L, anticorps smo
    Sujet
    Name/Gene ID: SMO
    Subfamily: Frizzled
    Family: GPCR

    Synonyms: SMO, Frizzled family member 11, FZD11, Gx, Protein Gx, SMOH, Smoothened, Smoothened homolog
    ID gène
    6608
    Pathways
    Signalisation Hedgehog
Vous êtes ici:
Support technique