Myc Tag anticorps (AA 410-419)

N° du produit ABIN1103152

Détail du produit anti-Myc Tag anticorps

Antigène: Myc Tag

Épitope: AA 410-419

Reactivité: Veuillez nous consulter SVP

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Myc Tag est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificité: This affinity purified antibody is directed against human c-Myc and is useful in determining its presence in various assays. This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Purification: Affinity purified

Stérilité: Sterile filtered

Immunogène: This antibody was purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide, E-Q-K-L-I-S-E-E-D-L, conjugated to KLH using maleimide. The sequence corresponds to amino acids 410-419 of human c-Myc.

Type of Immunogen: Purified protein

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA, IHC, WB (1:500 - 1:5000)

Usage: Anti-Myc has utility to detect the fusion protein of the Myc epitope cloned along with the target gene. As such, anti-Myc/Myc can be used to identify fusion proteins containing the Myc epitope. The antibody recognizes the Myc tag fused either to the AMINO- or CARBOXY- termini of targeted proteins. This antibody is suitable for ELISA and western blotting and was tested against both the immunizing peptide and Myc-tagged recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide, sterile filtered

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: avoid freeze thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Détails sur Myc Tag

Antigène: Myc Tag

Abstract: Myc Tag Produits

Classe de substances: Tag