His Tag anticorps
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- Antigène Voir toutes His Tag Anticorps
- His Tag
- Reactivité
- Veuillez nous consulter SVP
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp His Tag est non-conjugé
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Application
- Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
- Séquence
- HHHHHH
- Purification
- Affinity Chromatography.
- Immunogène
- Synthetic peptide containing the His epitope 6x His (H-H-H-H-H-H).
- Clone
- HIS-H8
- Isotype
- IgG2b
- Top Product
- Discover our top product His Tag Anticorps primaire
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS, pH 7.4 containing 0.05 % Sodium Azide as preservative.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- -20 °C
- Stockage commentaire
- Store the antibody (in aliquots) at -20 °C.
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- Antigène
- His Tag
- Autre désignation
- 6xHistidine Epitope Tag (His Tag Produits)
- Classe de substances
- Tag
- Sujet
- Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage, such expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. A system that has proven to be very successful relies on the insertion of a six histidine (6xHis) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni -chelating resins and purification by single step affinity chromatography. Visualization of such fusion proteins can be achieved by utilizing antibodies generated against specific peptide sequences downstream from the multiple cloning site. The antibody is useful for detection, isolation and localization of the His-tag proteins.Synonyms: 6xHis-Tag, HHHHHH Tag, HIS6 Tag, His Tag
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