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Vimentin anticorps (pSer55)

VIM Reactivité: Humain, Souris, Rat WB, IF Hôte: Souris Monoclonal 4A4 unconjugated
N° du produit ABIN1109485
  • Antigène Voir toutes Vimentin (VIM) Anticorps
    Vimentin (VIM)
    Épitope
    • 32
    • 17
    • 16
    • 16
    • 16
    • 14
    • 11
    • 8
    • 8
    • 7
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer55
    Reactivité
    • 333
    • 126
    • 125
    • 61
    • 59
    • 44
    • 43
    • 37
    • 30
    • 22
    • 20
    • 14
    • 13
    • 8
    • 7
    • 6
    • 6
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain, Souris, Rat
    Hôte
    • 190
    • 129
    • 9
    • 6
    • 4
    • 4
    • 2
    Souris
    Clonalité
    • 198
    • 145
    Monoclonal
    Conjugué
    • 200
    • 19
    • 13
    • 13
    • 11
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Cet anticorp Vimentin est non-conjugé
    Application
    • 282
    • 132
    • 99
    • 99
    • 96
    • 65
    • 49
    • 40
    • 39
    • 28
    • 23
    • 20
    • 8
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF)
    Séquence
    SLYSS-phosphoS55-PGGAYC-KLH
    Specificité
    This antibody reacts specifically with the phosphorylated MPV55 peptide but not the non-phosphorylated peptide. This antibody detects vimentin phosphorylated by cdc2 kinase and does not detect non-phosphorylated vimentin or phosphorylated vimentin by cAMP-dependent kinase, protein kinase C, or Ca2+-calmodulin-dependent protein kinase II on Western blotting.
    Réactivité croisée (Details)
    Species reactivity (tested):Human, mouse, rat
    Purification
    Protein A agarose
    Immunogène
    Synthetic MPV55 phosphopeptide corresponding to mouse phosphorylated vimentin Ser55.
    Clone
    4A4
    Isotype
    IgG2b
    Top Product
    Discover our top product VIM Anticorps primaire
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    PBS containing 50 % glycerol, pH 7.2. No preservative is contained.
    Agent conservateur
    Azide free
    Conseil sur la manipulation
    Avoid repeated freezing and thawing.
    Stock
    -20 °C
    Stockage commentaire
    Store (in aliquots) at -20 °C.
  • Antigène
    Vimentin (VIM)
    Autre désignation
    Vimentin (VIM Produits)
    Synonymes
    anticorps CTRCT30, anticorps cb28, anticorps vime, anticorps vim, anticorps vim1, anticorps vim2, anticorps VIM, anticorps Vimentin, anticorps vim4, anticorps vimentin, anticorps vimentin L homeolog, anticorps vimentin S homeolog, anticorps VIM, anticorps Vim, anticorps vim, anticorps vim.L, anticorps vim.S
    Sujet
    Vimentin is an intermediate filament protein distributed widely in the cytoplasm and is phosphorylated by several protein kinase in vitro. Ser55 residues on vimentin were reported to be one of the phosphorylation sites of vimentin at metaphase and were the phosphorylation sites for cdc2 kinase but not for cAMP-dependent protein kinase, protein kinase C, and Ca2+-calmodulin-dependent protein kinase II in vitro. Immunofluorescence and immunoelectron microscopy showed that vimentin Ser55 residues distributed in the entire cytoplasmic vimentin filament system are phosphorylated when the cells enter mitosis and de-phosphorylated in cytokinesis. The use of this antibody that specifically reacts with the phosphorylation site of vimentin Ser55 by cdc2 kinase enables estimation of a particular cdc2 kinase function.Synonyms: VIM
    ID gène
    7431
    NCBI Accession
    NP_003371
    UniProt
    P08670
    Pathways
    Caspase Cascade in Apoptosis
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