Cytokeratin 19 anticorps

N° du produit ABIN111167

Détail du produit anti-Cytokeratin 19 anticorps

Antigène: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Reactivité: Humain, Rat, Boeuf (Vache), Poulet, Amphibien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cytokeratin 19 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Séquence: VRADSERQNQ EYQRLMD Remarks: The epitope has been localized on amino acids 352-368 of the alpha-helical fragment.

Specificité: This antibody represents an excellent marker to discriminate glandular epithelial carcinoma from those of different origin. No reaction with hepatocellular Ca.! Recognizes a Mr 40,000 polypeptide (Cytokeratin 19) of Human glandular epithelia. Positive on the following cell lines: MCF-7, RT 112, Detroit 562, RPMI 2650, HT-29, SSC-12. Tumors Specifically Detected: All tested adenocarcinoma, cholangio ca. of liver, renal cell ca., transitional cell ca. of the bladder, ovary ca., squamous cell ca. of cervix, bronchus and lung (intermediate type), mesothelioma, carcinoid tumor of bronchus, breast ca., thymoma.

Réactivité croisée (Details): Species reactivity (tested):Human, Rat, Fish, Bovine.

Purification: Affinity Chromatography on Protein A .

Immunogène: Keratin K19 of Mr 40000 from cultured Human MCF-7 cells

Clone: KS19-2(Z105-6)

Isotype: IgG2b

Information d'application

Indications d'application: ELISA. Western blot. Cytological Material. Immunohistochemistry on Frozen Sections: Dilution Buffer: Dilute 1/10 with PBS, pH 7.4, for use in Immunohistochemistry. Incubation Time: 1 hour at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 1.0 mL distilled water.

Buffer: PBS pH 7.4, 0.09 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing. This reagent is not sterile, but can be sterilized by filtration if necessary. To minimize loss of volume during filtration, dilute to the final working concentration in the appropriate medium before filtration and filter through a 0.45µ Millipore filter (or equivalent).

Stock: -20 °C

Stockage commentaire: Store at -20 °C or below before reconstitution. Reconstitute with 0.5 ml of cold distilled water. After reconstitution, aliquot and freeze unused portions in volumes appropriate for single usage.

Détails sur Cytokeratin 19

Antigène: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Autre désignation: Cytokeratin 19 (KRT19 Produits)

Synonymes: anticorps CK19, anticorps K19, anticorps K1CS, anticorps AI663979, anticorps EndoC, anticorps Krt-1.19, anticorps Krt1-19, anticorps Ka19, anticorps k19, anticorps ck19, anticorps k1cs, anticorps krt9, anticorps krt15, anticorps MGC76282, anticorps GK-19, anticorps MGC83069, anticorps KRT19, anticorps keratin 19, anticorps keratin 19 L homeolog, anticorps keratin, type I cytoskeletal 19, anticorps KRT19, anticorps Krt19, anticorps krt19, anticorps krt19.L, anticorps LOC100344434, anticorps LOC101117946

Sujet: Cytokeratin 19 is a type I acidic subfamily of intermediate filament protein that is expressed in stratified and simple type epithelia. Keratin 19 is not expressed in hepatocytes, therefore, antibody to keratin 19 is useful in the identification of liver metastasis. The degree of keratin 19 positivity in breast cancer distinguishes malignant from benign tumors. Keratin 19 is often co-expressed with keratin 7.Synonyms: CK19, Cytokeratin-19, K19, KRT19, Keratin type I cytoskeletal 19, Keratin-19

ID gène: 3880

NCBI Accession: NP_002267

UniProt: P08727