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KRT20 anticorps (FITC)

KRT20 Reactivité: Humain, Rat, Porc IHC (p), IF, IHC (fro), FACS Hôte: Souris Monoclonal KS20-10 FITC
N° du produit ABIN111170
  • Antigène Voir toutes KRT20 Anticorps
    KRT20 (Keratin 20 (KRT20))
    Reactivité
    • 91
    • 29
    • 27
    • 9
    • 6
    • 2
    • 2
    • 1
    • 1
    Humain, Rat, Porc
    Hôte
    • 62
    • 30
    • 3
    • 1
    • 1
    Souris
    Clonalité
    • 52
    • 45
    Monoclonal
    Conjugué
    • 63
    • 12
    • 6
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Cet anticorp KRT20 est conjugé à/à la FITC
    Application
    • 68
    • 46
    • 25
    • 15
    • 14
    • 12
    • 11
    • 9
    • 6
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
    Specificité
    This Monoclonal antibody represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative. Polypeptide Recognized: Protein IT (keratin K20, Mr 46 000, also designated Cytokeratin 20). Tested Reactivities on Cultured Cell Lines: HT-29, LoVo, DLD-1, SW 1116, CaCo-2, RT-4.
    Aucune reactivité croisée
    Souris
    Réactivité croisée (Details)
    Species reactivity (tested):Human, Porcine (Pig) and Rat.
    Purification
    Affinity Chromatography
    Immunogène
    Electrophoretically purified Keratin K 20 from Human intestinal mucosa.
    Clone
    KS20-10
    Isotype
    IgG1
    Top Product
    Discover our top product KRT20 Anticorps primaire
  • Indications d'application
    FACS. Cytological Material. Immunohistochemistry on Frozen Sections. . Immunohistochemistry on Paraffin sections (Only after microwave treatment). Working Dilution: 1/10-1/20 in PBS, PH 7.4 for Immunohistochemistry. Incubation Time: 1h at RT or over night at 2-8 °C.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.2 mg/mL
    Conseil sur la manipulation
    Avoid repeated freezing and thawing.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Mușină, Zlei, Mentel, Scripcariu, Ștefan, Aniţei, Filip, Radu, Gavrilescu, Panuţa, Buna-Arvinte, Cordun, Predescu, Scripcariu, Huţanu: "Evaluation of circulating tumor cells in colorectal cancer using flow cytometry." dans: The Journal of international medical research, Vol. 49, Issue 9, pp. 300060520980215, (2021) (PubMed).

  • Antigène
    KRT20 (Keratin 20 (KRT20))
    Autre désignation
    Cytokeratin 20 (KRT20 Produits)
    Synonymes
    anticorps KRT20, anticorps CD20, anticorps CK-20, anticorps CK20, anticorps K20, anticorps KRT21, anticorps Krt21, anticorps 9030623C06Rik, anticorps keratin 20, anticorps KRT20, anticorps Krt20
    Sujet
    Cytokeratin 20 is a 46 kDa intermediate filament protein whose expression is restricted primarily to gastric and intestinal epithelium, urothelium, and Merkel cells. This antibody is highly specific to cytokeratin 20 and shows no cross-reactivity with other IFPs. Cytokeratin 20 is a unique type I keratin that is expressed in adenocarcinomas of the colon stomach, pancreas and bile system. It is also expressed in mucinous ovarian tumors, transitional cell carcinomas of the urinary tract, and Merkel cell carcinomas.Synonyms: CK20, Cytokeratin 20, K20, KRT20, Keratin type I cytoskeletal 20, Keratin-20, Protein IT
    ID gène
    54474
    NCBI Accession
    NP_061883
    UniProt
    P35900
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