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DYKDDDDK Tag anticorps

WB, IP, IF Hôte: Souris unconjugated
N° du produit ABIN1112984
  • Antigène Tous les produits DYKDDDDK Tag
    DYKDDDDK Tag
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    Hôte
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    Cet anticorp DYKDDDDK Tag est non-conjugé
    Application
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    Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
    Séquence
    DYKDDDDK
    Immunogène
    A synthetic peptide DYKDDDDK conjugated to KLH.
    Isotype
    IgG1
  • Indications d'application
    WB (1:1000-10000), IP (1:100-500), IF (1:200-1000). Other applications have not been tested. The optimal dilutions should be determined by end user.
    Restrictions
    For Research Use only
  • Validation #028889 (Immunofluorescence)
    'Independent Validation' signe
    by
    Reveal Biosciences
    No.
    #028889
    Date
    07.11.2013
    Antigène
    Numéro du lot
    L21/2013G
    Application validée
    Immunofluorescence
    Contrôle positif
    HEK293 cells transfected with DYKDDDDK Tag
    Contrôle négative
    Untransfected HEK293 cells
    Conclusion
    Signal was detected in positive control samples but not in negative control samples.
    'Independent Validation' signe
    Validation Images
    Protocole
    Anticorps primaire
    • Antibody: DYKDDDDK Tag antibody
    • Catalog number: ABIN1112984
    • Lot number: L21/2013G
    Anticorps secondaire
    • Antibody: Goat anti-mouse IgG (H+L) IF488 conjugated antibody
    • Lot number: S6/2013S
    Full Protocol
    • HEK293 cells were grown directly on coverslips and transfected with a DYKDDDDK tagged protein.
    • Cells were fixed with 4% paraformaldehyde in PBS for 15 min at room temperature (RT).
    • Fixed cells were rinsed three times in PBS for 5 min each at RT.
    • Cells were blocked in 1 X PBS / 5% normal goat serum / 0.3% Triton X-100 for 20 min at RT.
    • Cells were incubated with primary antibody diluted 1:100 in Universal Antibody Dilution Buffer (Electron
    • Microscopy Sciences, 25885-05) overnight at 4°C.
    • Cells were rinsed three times in PBS for 5 min each at RT.
    • Cells were incubated with secondary antibody diluted 1:150 in Universal Antibody Dilution Buffer
    • (Electron Microscopy Sciences, 25885-05) for 60 min at RT in dark.
    • Cells were rinsed three times in PBS for 5 min each at RT.
    • Coverslips were mounted on slides with Fluoro-Gel II with DAPI (Electron Microscopy Sciences Cat. No: 17985-50).
    • Stained cells were imaged with an EIDAQ 100 fluorescent microscope.
    Notes
    None
  • Format
    Liquid
    Buffer
    PBS (pH7.4), 50% Glycerol and 0.02% Sodium Azide.
    Agent conservateur
    Sodium azide, Thimerosal (Merthiolate)
    Stock
    4 °C
    Stockage commentaire
    Store at 2-8 °C for 1 month. Or aliquot and store at -20 °C or below for long term. Avoid repeated freeze and thaw cycles.
  • Antigène
    DYKDDDDK Tag
    Autre désignation
    Flag Tag (DYKDDDDK Tag Produits)
    Classe de substances
    Tag
    Sujet
    DYKDDDDK-Tag, or DYKDDDDK octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. The peptide sequence of the DYKDDDDK-Tag is: N-DYKDDDDK-C (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein.
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