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CD163 anticorps

CD163 Reactivité: Rat WB, FACS, IP, IHC (p), IF, IHC (fro) Hôte: Souris Monoclonal ED2 unconjugated
N° du produit ABIN111834
  • Antigène Voir toutes CD163 Anticorps
    CD163
    Reactivité
    • 140
    • 50
    • 30
    • 29
    • 20
    • 5
    • 4
    • 1
    Rat
    Hôte
    • 112
    • 52
    • 1
    Souris
    Clonalité
    • 96
    • 68
    • 1
    Monoclonal
    Conjugué
    • 56
    • 19
    • 14
    • 9
    • 7
    • 7
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp CD163 est non-conjugé
    Application
    • 115
    • 78
    • 54
    • 42
    • 27
    • 26
    • 26
    • 24
    • 22
    • 20
    • 15
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificité
    This antibody recognises the Rat CD163 cell surface glycoprotein, a 175 kDa molecule also known as ED2. It does not react with Human.
    Réactivité croisée (Details)
    Species reactivity (tested):Rat.
    Purification
    Affinity Chromatography on Protein A
    Immunogène
    Rat Spleen cell homogenate. Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0Ag 14 mouse myeloma cell line.
    Clone
    ED2
    Isotype
    IgG1
    Top Product
    Discover our top product CD163 Anticorps primaire
  • Indications d'application
    Western blot. Immunoprecipitation. Flow Cytometry: Use 10 μL of 1/10-1/100 diluted CD163 antibody to label 10^6 cells in 100μL. Immunohistochemistry on Frozen Sections: 1/50-1/100Immunohistochemistry on Paraffin Sections: This product requires antigen retrieval usingpronase prior to staining of paraffin sections. IHC on paraffin-embedded material following PLP fixation(Periodate-lysine-paraformaldehyde) - refer to Whiteland et al (Ref.5. )Histology Positive Control Tissue: liver.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    PBS with 0.09 % Sodium Azide as preservative
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid repeated freezing and thawing.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Antigène
    CD163
    Autre désignation
    CD163 (CD163 Produits)
    Synonymes
    anticorps DMBT1, anticorps CD163, anticorps CD163v2, anticorps CD163v3, anticorps M130, anticorps MM130, anticorps deleted in malignant brain tumors 1 protein-like, anticorps CD163 molecule, anticorps CD163 antigen, anticorps LOC426820, anticorps CD163, anticorps Cd163
    Sujet
    CD163 is a scavenger receptor for the haemoglobin-haptoglobin complex, and is upregulated by glucocorticoids and IL-10. The extracellular portion of the receptor is regularly shed and can be found in the circulation. An important function of CD163 seems to be in the adhesion of monocytes to activated endothelial cells. CD163 is expressed by approximately 50 % of peritoneal macrophages, a subset of splenic macrophages, and by macrophages in most other tissues. However, it is not expressed by monocytes, alveolar macrophages or microglial cells.Synonyms: Hemoglobin scavenger receptor, M130, Macrophage marker, Scavenger receptor cysteine-rich type 1 protein M130
    ID gène
    9332
    NCBI Accession
    NP_004235
    UniProt
    Q86VB7
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