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F4/80 anticorps (Biotin)

EMR1 Reactivité: Souris FACS, WB, IHC (p), IHC (fro) Hôte: Rat Monoclonal Cl:A3-1 Biotin
N° du produit ABIN111850
  • Antigène Voir toutes F4/80 (EMR1) Anticorps
    F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))
    Reactivité
    • 104
    • 52
    • 30
    • 4
    • 4
    • 1
    Souris
    Hôte
    • 67
    • 47
    • 4
    • 3
    • 2
    • 1
    Rat
    Clonalité
    • 67
    • 49
    • 2
    Monoclonal
    Conjugué
    • 43
    • 10
    • 10
    • 9
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp F4/80 est conjugé à/à la Biotin
    Application
    • 91
    • 56
    • 25
    • 18
    • 14
    • 13
    • 12
    • 12
    • 12
    • 6
    • 6
    • 3
    • 2
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    • 1
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    • 1
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    • 1
    • 1
    Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificité
    This antibody recognizes the Murine F4/80 antigen, a 160 kDa cell surface glycoprotein. Clone CI:A3-1 has been reported to modulate cytokine levels released in response to Listeria monocytogenes (Ref.5).
    Réactivité croisée (Details)
    Species reactivity (tested):Mouse.
    Purification
    Affinity Chromatography on Protein G.
    Immunogène
    Thioglycollate stimulated peritoneal macrophages from C57/BL mice. Spleen cells from immunised HOB2 rats were fused with cells of the mouse NS1 myeloma cell line.
    Clone
    Cl:A3-1
    Isotype
    IgG2b
    Top Product
    Discover our top product EMR1 Anticorps primaire
  • Indications d'application
    Flow Cytometry: Use 10 μL of 1/10-1/50 diluted antibody to stain 10^6 cells in 100 μL.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    PBS, pH 7.4 containing 1 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid repeated freezing and thawing.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Antigène
    F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))
    Autre désignation
    Macrophage F4/80 Antigen (EMR1 Produits)
    Synonymes
    anticorps EMR1, anticorps TM7LN3, anticorps DD7A5-7, anticorps EGF-TM7, anticorps F4/80, anticorps Gpf480, anticorps Ly71, anticorps adhesion G protein-coupled receptor E1, anticorps ADGRE1, anticorps LOC100424335, anticorps Adgre1
    Sujet
    F4/80 antigen is a member of the EGF-TM7 family of proteins which shares 68 % overall amino acid identity with human EGF module-containing mucinlike hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.Synonyms: Cell surface glycoprotein EMR1, EMR1 hormone receptor, Emr1, Gpf480
    ID gène
    13733
    NCBI Accession
    NP_034260
    UniProt
    Q61549
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