Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

CD163 anticorps

CD163 Reactivité: Humain, Singe WB, FACS, IHC (fro) Hôte: Souris Monoclonal RM3-1 unconjugated
N° du produit ABIN111878
  • Antigène Voir toutes CD163 Anticorps
    CD163
    Reactivité
    • 140
    • 50
    • 30
    • 29
    • 20
    • 5
    • 4
    • 1
    Humain, Singe
    Hôte
    • 112
    • 52
    • 1
    Souris
    Clonalité
    • 96
    • 68
    • 1
    Monoclonal
    Conjugué
    • 56
    • 19
    • 14
    • 9
    • 7
    • 7
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp CD163 est non-conjugé
    Application
    • 115
    • 78
    • 54
    • 42
    • 27
    • 26
    • 26
    • 24
    • 22
    • 20
    • 15
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificité
    This antibody is specific for CD163. Clone RM 3/1 recognizes a membrane glycoprotein on monocytes and macrophages which is expressed in intermediate and late inflammatory stages. Does not react with lymphocytes, granulocytes or platelets. Positive staining can be observed in the skin (histiocytes), gut, Kupffer cells, few alveolar macrophages, a major population of macrophages in the placenta, varying degrees of macrophages in inflamed tissues, including tumorous tissue depending on the inflammatory stage. Red pulp, but not white pulp macrophages of the spleen, and cortical macrophages of the thymus are detected. Macrophages in the synovialis of patients with rheumatoid arthritis. In alveolar macrophages and in Kupffer cells a double staining can be observed with clone 25F9 which is not the case in other tissues.
    Réactivité croisée (Details)
    Species reactivity (tested):Human, Monkey. Negative in Pig, Rabbit and Guinea Pig.
    Purification
    Affinity Chromatography
    Immunogène
    Human monocytes, particularly after dexamethasone treatment or after 2-5 days in culture
    Clone
    RM3-1
    Isotype
    IgG1
    Top Product
    Discover our top product CD163 Anticorps primaire
  • Indications d'application
    Immunohistochemistry on Frozen Sections: 4 μg/mL (1/100). Does not work in routinely processed paraffin sections. Has been described to work in FACS and Western blots. Recommended Positive control: Human placenta.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Reconstitution
    Restore with 0.5 mL distilled water to a concentration of 0.4 mg/mL.
    Concentration
    0.4 mg/mL
    Buffer
    Stock solutions contains PBS, pH 7.2, 10 mg/mL BSA as a stabilizer and 0.01 % Thimerosal as a preservative
    Agent conservateur
    Thimerosal (Merthiolate)
    Précaution d'utilisation
    This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    This product is photosensitive and should be protected from light
    Stock
    4 °C
    Stockage commentaire
    Store the antibody undiluted Prior to and after reconstitution at 2-8 °C. DO NOT FREEZE!
  • Antigène
    CD163
    Autre désignation
    CD163 (CD163 Produits)
    Synonymes
    anticorps DMBT1, anticorps CD163, anticorps CD163v2, anticorps CD163v3, anticorps M130, anticorps MM130, anticorps deleted in malignant brain tumors 1 protein-like, anticorps CD163 molecule, anticorps CD163 antigen, anticorps LOC426820, anticorps CD163, anticorps Cd163
    Sujet
    CD163 is a scavenger receptor for the haemoglobin-haptoglobin complex, and is upregulated by glucocorticoids and IL-10. The extracellular portion of the receptor is regularly shed and can be found in the circulation. An important function of CD163 seems to be in the adhesion of monocytes to activated endothelial cells. CD163-positive cells include skin histiocytes, Kupffer cells, spleen macrophages of the red pulp, and some thymus macrophages. The antigen is also found abundantly in human term placenta, and regularly in acute and chronic inflammatory lesions.Synonyms: Hemoglobin scavenger receptor, M130, Macrophage marker, Scavenger receptor cysteine-rich type 1 protein M130
    ID gène
    9332
    NCBI Accession
    NP_004235
    UniProt
    Q86VB7
Vous êtes ici:
Support technique