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TNFRSF1B anticorps

TNFRSF1B Reactivité: Humain WB, FACS, IP, IHC (fro), IHC (p) Hôte: Souris Monoclonal utr1 unconjugated
N° du produit ABIN111940
  • Antigène Voir toutes TNFRSF1B Anticorps
    TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))
    Reactivité
    • 87
    • 62
    • 34
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Humain
    Hôte
    • 62
    • 31
    • 13
    • 5
    • 4
    • 1
    • 1
    Souris
    Clonalité
    • 62
    • 55
    Monoclonal
    Conjugué
    • 69
    • 19
    • 9
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp TNFRSF1B est non-conjugé
    Application
    • 69
    • 44
    • 33
    • 33
    • 32
    • 24
    • 19
    • 17
    • 14
    • 9
    • 7
    • 6
    • 5
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Purification
    Affinity Chromatography
    Immunogène
    Partially purified TNF binding proteins
    Clone
    Utr1
    Isotype
    IgG1
    Top Product
    Discover our top product TNFRSF1B Anticorps primaire
  • Indications d'application
    Immunohistochemistry on Frozen Sections: 5 μg/mL (1/200). Immunohistochemistry on Paraffin Sections: 50 μg/mL (1/20). Pretreatment not necessary. Has been described to work in FACS, Immunoprecipitation and Western Blots. Suggested Positive Control: Human tonsil.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Protocole
    Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalumProtocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature
    Restrictions
    For Research Use only
  • Reconstitution
    Restore with 0.5 mL distilled water
    Concentration
    1.0 mg/mL
    Buffer
    PBS, pH 7.2, 0.05 % Kathon CG, 5 mg/mL BSA
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid repeated freezing and thawing.
    Stock
    -20 °C
  • Antigène
    TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))
    Autre désignation
    CD120b / TNFR2 (TNFRSF1B Produits)
    Synonymes
    anticorps CD120b, anticorps TBPII, anticorps TNF-R-II, anticorps TNF-R75, anticorps TNFBR, anticorps TNFR1B, anticorps TNFR2, anticorps TNFR80, anticorps p75, anticorps p75TNFR, anticorps TNF-RII/TNFRSF1B, anticorps TNFR-II, anticorps TNFRSF1B, anticorps TNF-R2, anticorps TNF-alphaR2, anticorps TNFRII, anticorps TNFalpha-R2, anticorps Tnfr-1, anticorps Tnfr2, anticorps rank, anticorps zgc:163064, anticorps TNF receptor superfamily member 1B, anticorps tumor necrosis factor receptor superfamily, member 1b, anticorps tumor necrosis factor receptor superfamily, member 1B, anticorps TNFRSF1B, anticorps Tnfrsf1b, anticorps tnfrsf1b
    Sujet
    Tumor Necrosis Factor (TNF) is a cytokine whose function is mediated through two distinct cell surface receptors (TNF Receptor I and TNF Receptor II) that are included in the TNF receptor superfamily along with FAS antigen and CD40. TNF receptors I and II are membrane glycoproteins of 55 and 75 kDa respectively. They are from the family of cell surface molecules including nerve growth factor receptor, Fas/Apo1, CD30, OX40, and 4-1BB, which are characterized by cysteine rich motifs in the extracellular domain. TNF Receptor II (p75, CD120b) is present on most cell types (including monocytes, endothelial cells, Langerhans cells, and macrophages) and is considered to play a role in cell stimulation by TNF alpha. TNF Receptor II molecule is shown to be responsible for stimulation of activated T lymphocytes by TNF alpha.Synonyms: TNF-R2, TNFBR, TNFRSF1B, Tumor necrosis factor receptor 2, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor type II, p80 TNF-alpha receptor
    ID gène
    9606
    UniProt
    P20333
    Pathways
    Signalisation NF-kappaB, Apoptose, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Ubiquitin Proteasome Pathway
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