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TNFRSF1B anticorps

TNFRSF1B Reactivité: Humain WB, FACS, IP, Func, IHC (fro) Hôte: Souris Monoclonal utr1 unconjugated
N° du produit ABIN111942
  • Antigène Voir toutes TNFRSF1B Anticorps
    TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))
    Reactivité
    • 86
    • 62
    • 34
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Humain
    Hôte
    • 62
    • 30
    • 13
    • 5
    • 4
    • 1
    • 1
    Souris
    Clonalité
    • 61
    • 55
    Monoclonal
    Conjugué
    • 68
    • 19
    • 9
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp TNFRSF1B est non-conjugé
    Application
    • 68
    • 43
    • 33
    • 32
    • 32
    • 23
    • 18
    • 17
    • 14
    • 9
    • 7
    • 6
    • 5
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Réactivité croisée (Details)
    Species reactivity (tested):Human.
    Purification
    Affinity Chromatography on Protein G.
    Immunogène
    Partially purified preparations of TNF binding proteins from U937 cells.
    Clone
    Utr1
    Isotype
    IgG1
    Top Product
    Discover our top product TNFRSF1B Anticorps primaire
  • Indications d'application
    Immunohistology: 2-5 μg/mL on Cryostat Sections (2 % Periodate-lysine-paraformaldehydeor acetone for fixation). Flow Cytometry: 10 μg/mL. Immunoprecipitation. Western blots: 30 μg/mL on non-reduced SDS PAGE, the epitope is sensitive to reduction,may need enrichment by Immunoprecipitation. Functional Assay: This antibody utr1 is useful for studying biological effects ofTNF-Receptor p75 in vitro where it inhibits the binding of radiolabelled TNF to human cellsexpressing the p75 TNF receptor. In order to completely block TNF binding to the cellsurface, 10 μg/mL of utr1 are required. utr1 itself may have an agonistic effect in assaysmeasuring cytotoxicity, fibroblast growth or IL-6 secretion.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Protocole
    Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum
    Restrictions
    For Research Use only
  • Concentration
    2.0 mg/mL (by Absorbanece at 280 nm)
    Buffer
    PBS, pH 7.2, None, None
    Agent conservateur
    Without preservative
    Conseil sur la manipulation
    Avoid repeated freezing and thawing.
    Stock
    -20 °C/-80 °C
    Stockage commentaire
    Store lyophilized (preferably in a desiccator) at -20 °C and in aliquots at -80 °C. Reconstituted antibody can be stored at 4 °C for a limited period of time, it does not show decline in activity after two weeks at 4 °C.
  • Antigène
    TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))
    Autre désignation
    CD120b / TNFR2 (TNFRSF1B Produits)
    Synonymes
    anticorps CD120b, anticorps TBPII, anticorps TNF-R-II, anticorps TNF-R75, anticorps TNFBR, anticorps TNFR1B, anticorps TNFR2, anticorps TNFR80, anticorps p75, anticorps p75TNFR, anticorps TNF-RII/TNFRSF1B, anticorps TNFR-II, anticorps TNFRSF1B, anticorps TNF-R2, anticorps TNF-alphaR2, anticorps TNFRII, anticorps TNFalpha-R2, anticorps Tnfr-1, anticorps Tnfr2, anticorps rank, anticorps zgc:163064, anticorps TNF receptor superfamily member 1B, anticorps tumor necrosis factor receptor superfamily, member 1b, anticorps tumor necrosis factor receptor superfamily, member 1B, anticorps TNFRSF1B, anticorps Tnfrsf1b, anticorps tnfrsf1b
    Sujet
    Tumor Necrosis Factor (TNF) is a cytokine whose function is mediated through two distinct cell surface receptors (TNF Receptor I and TNF Receptor II) that are included in the TNF receptor superfamily along with FAS antigen and CD40. TNF receptors I and II are 55 and 75 kDa members, respectively, of a family of cell surface molecules including nerve growth factor receptor, Fas/Apo1, CD30, OX40, and 4-1BB, which are characterized by cysteine rich motifs in the extracellular domain. TNF Receptor II (p75, CD120b) is present on most cell types (including monocytes, endothelial cells, Langerhans cells, and macrophages) and is considered to play a role in cell stimulation by TNF alpha. TNF Receptor II molecule is shown to be responsible for stimulation of activated T lymphocytes by TNF alpha.Synonyms: TNF-R2, TNFBR, TNFRSF1B, Tumor necrosis factor receptor 2, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor type II, p80 TNF-alpha receptor
    ID gène
    9606
    UniProt
    P20333
    Pathways
    Signalisation NF-kappaB, Apoptose, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Ubiquitin Proteasome Pathway
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