hCG anticorps

N° du produit ABIN111952

Détail du produit anti-hCG anticorps

Antigène: hCG (Chorionic Gonadotropin (hCG))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp hCG est non-conjugé

Application: Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: This Monoclonal antibody Clone h6 recognizes β-HCG, a well defined oncofetal protein of 36.7 kDa m.w. associated with pregnancy and some cancers. Raised serum levels for HCG are found in teratogenic carcinomas and trophoblastic tumors and occasionally in gastrointestinal, urogenital, bronchial or mammary carcinomas, and in lymphomas.

Purification: Affinity Chromatography

Clone: H6

Isotype: IgG1

Information d'application

Indications d'application: Suitable for Immunohistochemistry (IHC) on: Frozen sections: 0.1 μg/mL (1/4000)Paraffin sections: 0.1 μg/mL (1/4000), microwave pretreatment for antigen retrieval isrecommended. It works also in ELISA. Suggested positive control: Human placenta.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 0.4 mg/mL

Buffer: PBS, pH 7.2 with 10 mg/mL BSA as a stabilizer and 0.01 % Thimerosal as a preservative

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Détails sur hCG

Antigène: hCG (Chorionic Gonadotropin (hCG))

Abstract: hCG Produits

Synonymes: anticorps CGB, anticorps AW108023, anticorps HCG, anticorps chorionic gonadotropin, beta polypeptide, anticorps ubiquitin-like 3, anticorps chorionic gonadotropin beta subunit 5, anticorps CGB, anticorps Ubl3, anticorps CGB5

Sujet: Human gonadothropin is a glycoprotein of 36.7 kDa consisting of two subunits. The α-chain (MW 14.5 kD) is common to three other glycoprotein hormones (TSH, FSH, LH) whereas the β-chain (22.2kD) is more different. The β-chain of HCG and LH (lutrophin) have sequence homology in the first 112 amino acids and differ at the C-terminal end consisting of 24 amino acids.Synonyms: CGB, CGB3, Choriogonadotropin subunit beta, beta hCG, hCG-beta

ID gène: 1082

NCBI Accession: NP_000728

UniProt: P01233