Cytokeratin 5 anticorps

N° du produit ABIN112167

Détail du produit anti-Cytokeratin 5 anticorps

Antigène: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Reactivité: Humain, Souris, Boeuf (Vache), Porc, Hydra, Amphibien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cytokeratin 5 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: Antibody clone C22 represents an excellent marker for distinguishing carcinomas from all non-epithelial tumors. The antibody specifically reacts with keratins K5 and K8 present in nearly all epithelia. Polypeptide reacting: Mr 52 500, Mr 58 000 keratins (type II keratins K5 and K8, formerly also designated cytokeratins 5 and 8) of human epithelial cells. Epitope has been mapped to aa 353-367 on alpha helical rod domain (see Waseem et al., 2004). Reactivities on cultured cell lines (tested so far): MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650, SSC-12, bovine BMGE+H, BMGE-H, MDBK.

Réactivité croisée (Details): Also reacts to: KRT8

Purification: Affinity Chromatography on Protein A.

Immunogène: Human keratin K8, purified from SDS PAGE gel

Clone: C22

Isotype: IgG1

Information d'application

Indications d'application: Western Blot. Immunocytology. Immunohistochemistry on Frozen Tissues (1/10), Incubation time: 1 h at RT. Immunohistochemistry on Paraffin-Embedded Tissues (1/10), Incubation time: overnight at2-8 °C. With paraffin-embedded sections, protease pretreatment is required prior toantibody application.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in 1 mL distilled water.

Buffer: Final solution contains 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer in PBS buffer, pH 7.4.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: This product is photosensitive and should be protected from light. DO NOT FREEZE!

Stock: 4 °C

Stockage commentaire: Prior to and following reconstitution store the antibody undiluted at 2-8 °C.

Détails sur Cytokeratin 5

Antigène: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Autre désignation: Cytokeratin 5 (KRT5 Produits)

Synonymes: anticorps 3300001P10Rik, anticorps AW146334, anticorps CK5, anticorps K5, anticorps Krt2-5, anticorps Tfip8, anticorps DDD, anticorps DDD1, anticorps EBS2, anticorps KRT5A, anticorps Sak57, anticorps ckii, anticorps wu:fb02h09, anticorps wu:fj02d02, anticorps wu:fk78b12, anticorps zfCKII, anticorps KRT75, anticorps CK-5, anticorps Keratin-5, anticorps keratin 5, anticorps Krt5, anticorps KRT5, anticorps krt5

Sujet: Cytokeratin 5 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. Cytokeratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.Synonyms: 58 kDa Cytokeratin, CK5, Cytokeratin-5, K5, KRT5, Keratin 5, Keratin type II cytoskeletal 5, Keratin-5

ID gène: 3852

NCBI Accession: NP_000415

UniProt: P13647