Keratin 6 anticorps

N° du produit ABIN112170

Détail du produit anti-Keratin 6 anticorps

Antigène: Keratin 6 (KRT6)

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Keratin 6 est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: The antibody is suitable for discrimination of keratinizing and non-keratinizing squamous cell carcinoma versus e. g. poorly differentiated adenocarcinoma. Clone Ks6.KA12 represents an excellent marker for non-keratinized squamous epithelia and proliferating cells of epidermis (e.g. within psoriatic lesions). Reacts with a Mr 56000 polypeptide (keratin K6, formerly also designated cytokeratin 6) of squamous epithelia. Tested Reactivities on Cultured Cell Lines: A-431, Detroit 562 and SCC-12.

Purification: Affinity Chromatography on Protein A.

Immunogène: Keratin K6 of Human callus cytoskeletal preparation.

Clone: Ks6-KA12

Isotype: IgG1

Information d'application

Indications d'application: Immunoblotting (Western blot). Immunohistochemistry on Frozen and Paraffin Embedded Sections after formalin fixation(microwave treatment improves performance). After reconstitution, dilute the antibodyfurther 1/10 with PBS for Immunohistochemical applications. Incubation Time: 1h at RT, extended with Paraffin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in 1 mL distilled water.

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Références pour anticorps anti-Keratin 6 (ABIN112170)

Poschmann, Sitek, Sipos, Ulrich, Wiese, Stephan, Warscheid, Klöppel, Vander Borght, Ramaekers, Meyer, Stühler: "Identification of proteomic differences between squamous cell carcinoma of the lung and bronchial epithelium." dans: Molecular & cellular proteomics : MCP, Vol. 8, Issue 5, pp. 1105-16, (2009) (PubMed).

Détails sur Keratin 6

Antigène: Keratin 6 (KRT6)

Autre désignation: Cytokeratin 6 (KRT6 Produits)

Synonymes: anticorps Krt2-6, anticorps Krt-2.6, anticorps keratin 6, anticorps Krt6

Sujet: Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyperproliferation related keratins). Keratin 6 is found in hair follicles, neck squamous cell carcinomas, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyperproliferative situations. Epidermal injury results in activation of keratinocytes which express CK6 and CK16. CK6 is strongly expressed in about 75 % of head and neck squamous cell carcinomas. Expression of CK6 is particularly associated with differentiation. There are at least six isoforms of human type II keratin 6 (K6), K6A being the most abundant representing about 77 % of all forms found in epithelia.Synonyms: Cytokeratin 6, Cytokeratin6A, Cytokeratin6D, K6, KRT-6, KRT6A, KRT6B, KRT6C, KRT6D, KRT6E, KRTL1, Keratin type II cytoskeletal 6A

ID gène: 3853

NCBI Accession: NP_005545

UniProt: P02538