Desmocollin 3 anticorps

N° du produit ABIN112239

Détail du produit anti-Desmocollin 3 anticorps

Antigène: Desmocollin 3 (DSC3)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Desmocollin 3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Séquence: VHGAPFYFSL PNTSPEISRL WSLTKVC

Specificité: This anibody localizes Dsc3 present in: - all living epidermal layers - in glandular duct cells - in basal matrix cells and outer root sheath of hair follicles - basal and suprabasal cell layers of stratified epithelia (e.g. vagina, tongue, esophagus), basal layer of bladder urothelium and complex epithelium of trachea - thymic reticulum cells All simple epithelia are negative. Intercalated disks of myocardium are negative. Polypeptide reacting: 109 kDa and 100 kDa polypeptides (desmocollin 3 splice isoforms at pI 5.2) from human epidermal desmosomes. Most tested cultured cell lines derivated from stratified squamous epithelia or squamous cell carcinoma are positive (e.g A-431, HaCat). Does not react with Bovine and Rodent.

Purification: Affinity Chromatography on Protein A

Immunogène: Synthetic peptide corresponding to a sequence present on the extracellular anchor domain of Human Desmocollin 3.

Clone: Dsc3-U114

Isotype: IgG1

Information d'application

Indications d'application: Immunoblotting (Western). Immunohistochemistry on Frozen Sctions. Immunohistochemistry on Paraffin-Embedded Sections after microwave treatment: 1/10 inPBS ( pH 7.4) for 1h at RT. Recommendations: For better resolution on Frozen Sections preincubation (directly afterfixation) with 0.05-0.2 % Triton X100, for 5-10 min, depending on tissue type, isrecommended (Ref.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in 1 mL distilled water.

Buffer: PBS buffer, pH 7.4, 0.09 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: This product is photosensitive and should be protected from light

Stock: 4 °C

Stockage commentaire: Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Références pour anticorps anti-Desmocollin 3 (ABIN112239)

Lorén, Cabré, Ojanguren, Domènech, Pedrosa, García-Jaraquemada, Mañosa, Manyé et al.: "Interleukin-10 Enhances the Intestinal Epithelial Barrier in the Presence of Corticosteroids through p38 MAPK Activity in Caco-2 Monolayers: A Possible Mechanism for Steroid Responsiveness in ..." dans: PLoS ONE, Vol. 10, Issue 6, pp. e0130921, (2016) (PubMed).

Brenner, Riha, Giessrigl, Thalhammer, Grusch, Krupitza, Stieger, Jäger: "The effect of organic anion-transporting polypeptides 1B1, 1B3 and 2B1 on the antitumor activity of flavopiridol in breast cancer cells." dans: International journal of oncology, Vol. 46, Issue 1, pp. 324-32, (2015) (PubMed).

Hamidov, Altendorf-Hofmann, Chen, Settmacher, Petersen, Knösel: "Reduced expression of desmocollin 2 is an independent prognostic biomarker for shorter patients survival in pancreatic ductal adenocarcinoma." dans: Journal of clinical pathology, Vol. 64, Issue 11, pp. 990-4, (2012) (PubMed).

Cui, Chen, Yang, Mireskandari, Knösel, Zhang, Kohler, Kunze, Presselt, Petersen: "Diagnostic and prognostic impact of desmocollins in human lung cancer." dans: Journal of clinical pathology, Vol. 65, Issue 12, pp. 1100-6, (2012) (PubMed).

Goris, Uhlenbruck, Schwegmann-Wessels, Köhl, Niedorf, Stern, Hewicker-Trautwein, Bals, Taylor, Braun, Bicker, Kietzmann, Herrler: "Differential sensitivity of differentiated epithelial cells to respiratory viruses reveals different viral strategies of host infection." dans: Journal of virology, Vol. 83, Issue 4, pp. 1962-8, (2009) (PubMed).

Détails sur Desmocollin 3

Antigène: Desmocollin 3 (DSC3)

Autre désignation: Desmocollin-3 (DSC3 Produits)

Synonymes: anticorps CDHF3, anticorps DSC, anticorps DSC1, anticorps DSC2, anticorps DSC4, anticorps HT-CP, anticorps 5430426I24Rik, anticorps MGC85083, anticorps desmocollin 3, anticorps desmocollin 3 S homeolog, anticorps DSC3, anticorps Dsc3, anticorps dsc3.S, anticorps dsc3

Sujet: Desmocollin 3 is a calcium-dependent glycoprotein that is a member of the desmocollin subfamily of the cadherin superfamily. These desmosomal family members, along with the desmogleins, are found primarily in epithelial cells where they constitute the adhesive proteins of the desmosome cell-cell junction and are required for cell adhesion and desmosome formation. The desmosomal family members are arranged in two clusters on chromosome 18, occupying less than 650 kb combined.Synonyms: CDHF3, Cadherin family member 3, DSC3, DSC4, Desmocollin-4, HT-CP

ID gène: 1825

NCBI Accession: NP_001932

UniProt: Q14574