Transferrin anticorps (N-Term)

N° du produit ABIN112496

Détail du produit anti-Transferrin anticorps

Antigène: Transferrin (TF)

Épitope: N-Term

Reactivité: Humain, Rat, Porc, Lapin

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Transferrin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Functional Studies (Func)

Specificité: This antibody is specific for the N-terminal domain of Transferrin and is known to block the binding of this antibody to Transferrin. HTF-14 recognises a band of approximately 77 kDa under reducing conditions in Human plasma. Clone HTF-14 is not able to recognize receptor bound Transferrin. Clone HTF-14 recognizes a conformational epitope in a loop of the N lobe of Transferrin involving the critical Lys-144 residue, also essential for binding of the N lobe to the transferrin receptor (Mason et al.2009) Transferrin is a 77 kDa plasma protein synthesized by the liver involved with the transport of iron. Each transferrin molecule has two domains, both with facility to carry 2 ferric ions. The iron/transferrin complex is essential for haemoglobin synthesis and certain types of cell division. Negative Species. Bovine, Sheep, Canine (Dog) and Equine (Horse).

Réactivité croisée (Details): Species reactivity (expected):Porcine, Rat and Rabbit.
Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein A

Immunogène: Purified Porcine Transferrin.

Clone: HTF-14

Isotype: IgG1

Information d'application

Indications d'application: ELISA. Immunofluorescence. Functional Application.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Transferrin

Antigène: Transferrin (TF)

Abstract: TF Produits

Synonymes: anticorps ltf, anticorps pro1557, anticorps pro2086, anticorps mgc107777, anticorps LOC692564, anticorps TF, anticorps LOC100144362, anticorps tf, anticorps LTF, anticorps TFEW, anticorps conalbumin, anticorps tf-b, anticorps MGC64306, anticorps PRO1557, anticorps PRO2086, anticorps TFQTL1, anticorps AI266983, anticorps Cd176, anticorps HP, anticorps Tf, anticorps Tfn, anticorps hpx, anticorps Trf, anticorps cb285, anticorps gavi, anticorps id:ibd3238, anticorps id:ibd3525, anticorps sb:cb285, anticorps wu:fb57g06, anticorps wu:fb62h02, anticorps wu:fb63h10, anticorps wu:fb64h10, anticorps zgc:112154, anticorps 143958_at, anticorps CG6186, anticorps Dmel\\CG6186, anticorps TSF1, anticorps anon-EST:Posey265, anticorps tsf1, anticorps Pro-TRH, anticorps IL-5, anticorps STF I, anticorps TRF1, anticorps sTF1, anticorps sTf, anticorps tf1, anticorps transferrin, anticorps serotransferrin, anticorps transferrin (ovotransferrin), anticorps transferrin L homeolog, anticorps melanotransferrin, anticorps transferrin-a, anticorps Transferrin 1, anticorps thyrotropin releasing hormone, anticorps interleukin 5, anticorps TF, anticorps LOC477072, anticorps tf, anticorps Tf, anticorps LOC100144362, anticorps tf.L, anticorps LOC5575625, anticorps Trf, anticorps tfa, anticorps Tsf1, anticorps TRH, anticorps IL5, anticorps LOC101085148, anticorps LOC100726872, anticorps trf

Sujet: Transferrin is a monomeric glycoprotein of approximately 77 kDa, which serves as an iron-transporter. In normal plasma, transferrin has a concentration of 25-50 mmol / liter, and is usually about one-third saturated with iron, thus providing a large buffering capacity in case of an acute increase in plasma iron levels. Cells take up transferrin-iron complexes (holotransferrin) using transferrin receptor dimers. Upon binding of holotransferrin, the receptor is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with its receptor (CD71) and recycles to the cell surface, where apotransferrin is released upon exposure to normal pH . Internalization of labeled transferrin thus represents an usefull approach to study endocytosis. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. Iron/transferrin complex is essential in haemoglobin synthesis and for certain types of cell division.Synonyms: Beta-1 metal-binding globulin, Serotransferrin, Siderophilin

ID gène: 7018

NCBI Accession: NP_001054

UniProt: P02787

Pathways: Transition Metal Ion Homeostasis