LDLR anticorps (Extracellular Domain)

N° du produit ABIN113461

Détail du produit anti-LDLR anticorps

Antigène: LDLR (Low Density Lipoprotein Receptor (LDLR))

Épitope: AA 184-195, Extracellular Domain

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LDLR est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: The antibody reacts specifically with The LDL Receptor (160 kDa mature or glycosylated receptor, 120 kDa precursor or unglycosylated receptor) plays a key role in cellular cholesterol homeostasis. It does not inhibit binding of LDL.

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography

Immunogène: Specific synthetic peptide (sequence not conserved in VLDL receptor and LRP) of the LDL receptor extracellular domain (epitope between residues 184-195, the linker region between repeats 4 and 5)

Information d'application

Indications d'application: Flow Cytometry: 1/10. Immunobloting: 1/100-1/200. Recognizes the 160 kDa band of LDLR and a 120 kDa band ofthe LDLR precursor from Fibroblasts, Hepatocytes, and monocytic cells cultured in thepresence of lipoprotein-deficient serum. Immunohistochemistry: 1/20. Receptor Binding Studies.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.5 % BSA

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the lyophilized antibody at -20 °C. Following reconstitution, the product ist stable at 2-8 °C for up one week or (in aliquots) at -20 °C for up to six months.

Références pour anticorps anti-LDLR (ABIN113461)

Akazawa, Date, Morikawa, Murayama, Miyamoto, Kaga, Barth, Baumert, Dubuisson, Wakita: "CD81 expression is important for the permissiveness of Huh7 cell clones for heterogeneous hepatitis C virus infection." dans: Journal of virology, Vol. 81, Issue 10, pp. 5036-45, (2007) (PubMed).

Détails sur LDLR

Antigène: LDLR (Low Density Lipoprotein Receptor (LDLR))

Autre désignation: LDLR (LDLR Produits)

Synonymes: anticorps FH, anticorps FHC, anticorps LDLCQ2, anticorps Hlb301, anticorps LDLRA, anticorps LDLA, anticorps LDL receptor-2, anticorps fhc, anticorps ldlcq2, anticorps ldlr, anticorps ldlr2-a, anticorps LDL receptor 1, anticorps ldlr-a, anticorps ldlr-b, anticorps low density lipoprotein receptor, anticorps low density lipoprotein receptor a, anticorps low density lipoprotein receptor S homeolog, anticorps low density lipoprotein receptor L homeolog, anticorps LDLR, anticorps Ldlr, anticorps ldlra, anticorps ldlr.S, anticorps ldlr.L

Sujet: The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemiaSynonyms: LDL receptor, LDLR, Low-density lipoprotein receptor

ID gène: 3949

NCBI Accession: NP_000518

UniProt: P01130

Pathways: Hepatitis C, Lipid Metabolism