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ITGA4 anticorps (Biotin)

ITGA4 Reactivité: Souris FACS, IHC (fro) Hôte: Rat Monoclonal R1-2 Biotin
N° du produit ABIN114224
  • Antigène Voir toutes ITGA4 Anticorps
    ITGA4 (Integrin alpha 4 (ITGA4))
    Reactivité
    • 140
    • 104
    • 36
    • 19
    • 14
    • 10
    • 9
    • 8
    • 8
    • 7
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Hôte
    • 81
    • 56
    • 48
    Rat
    Clonalité
    • 117
    • 70
    • 1
    Monoclonal
    Conjugué
    • 84
    • 18
    • 17
    • 14
    • 7
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp ITGA4 est conjugé à/à la Biotin
    Application
    • 113
    • 62
    • 43
    • 40
    • 32
    • 25
    • 13
    • 11
    • 10
    • 6
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificité
    This monoclonal antibody reacts with a4 integrin, which helps to mediate cell-cell and cell-matrix interactions.
    Purification
    Protein G Chromatography
    Immunogène
    Peyers Patch HEV binding lymphoma line (TK1) Donor: Fisher Spleen Fusion Partner: P3x63Ag8.653
    Clone
    R1-2
    Isotype
    IgG2b
    Top Product
    Discover our top product ITGA4 Anticorps primaire
  • Indications d'application
    Flow cytometry. Immunoprecipitation. Immunohistochemistry. (1,2,3)
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Protocole
    FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add ~1. 0 µg* of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (Streptavidin-FITC) at a 1: 500 dilution. 9. Incubate tubes at 4°C for 30 - 60 minutes (It is recommended that tubes are protectedfrom light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results - Tissue Distribution: Mouse Strain: BALB/cCell Concentration: 1x10e6 cells per testsAntibody Concentration Used: 1. 0 µg/10e6 cellsIsotypic Control: Biotin Rat IgG2bCell Source: Percentage of cells stained above control: TK1 Cells: 97. 9%Thymus: 80. 0%Spleen: 85. 1%Bone Marrow: 72. 4%
    Restrictions
    For Research Use only
  • Concentration
    0.1 mg/mL
    Buffer
    PBS, 0.02 % NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid repeated freezing and thawing.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Antigène
    ITGA4 (Integrin alpha 4 (ITGA4))
    Autre désignation
    CD49d / ITGA4 (ITGA4 Produits)
    Synonymes
    anticorps CD49D, anticorps IA4, anticorps LOC100219853, anticorps integrin subunit alpha 4, anticorps integrin alpha 4, anticorps integrin subunit alpha 4 S homeolog, anticorps ITGA4, anticorps Itga4, anticorps itga4.S
    Sujet
    Integrin alpha 4 (also called CD49d) is a 150 kDa protein that possesses a large extracellular domain involved in ligand binding, a single transmembrane domain, and an intracellular regulatory domain possessing multiple sites for phosphorylation. Integrin alpha 4 forms heterodimers with integrins beta 1 and beta 7. Integrin alpha 4 is expressed on leukocytes and leukocyte precursors, neural crest cells, and developing skeletal muscles and is essential for embryogenesis, hematopoiesis, and immune responses. The presence of integrin alpha 4 promotes cell migration and inhibits cell spreading and contractility. Integrin alpha 4 function has been implicated in the pathogenesis of multiple diseases including asthma, rheumatoid arthritis, Crohn's disease, ulcerative colitis, hepatitis C, and multiple sclerosis, and therefore, modulation of integrin alpha 4 function has become an important target for drug discovery.Synonyms: CD49 antigen-like family member D, Integrin alpha-4, Integrin alpha-IV, VLA-4, VLA4
    ID gène
    16401
    NCBI Accession
    NP_034706
    UniProt
    Q00651
    Pathways
    Integrin Complex
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