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ITGA4 anticorps

ITGA4 Reactivité: Souris FACS, IP, IHC (fro), Func Hôte: Rat Monoclonal R1-2 unconjugated
N° du produit ABIN114228
  • Antigène Voir toutes ITGA4 Anticorps
    ITGA4 (Integrin alpha 4 (ITGA4))
    Reactivité
    • 140
    • 103
    • 36
    • 19
    • 14
    • 10
    • 9
    • 8
    • 8
    • 7
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Hôte
    • 81
    • 56
    • 47
    Rat
    Clonalité
    • 116
    • 70
    • 1
    Monoclonal
    Conjugué
    • 83
    • 18
    • 17
    • 14
    • 7
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp ITGA4 est non-conjugé
    Application
    • 112
    • 62
    • 43
    • 40
    • 31
    • 25
    • 12
    • 10
    • 10
    • 6
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)
    Specificité
    This antibody reacts with alpha 4 integrin.
    Purification
    Protein G affinity purified immunoglobulin fraction
    Immunogène
    Peyers Patch HEV binding lymphoma line (TK1)
    Clone
    R1-2
    Isotype
    IgG2b
    Top Product
    Discover our top product ITGA4 Anticorps primaire
  • Indications d'application
    Flow cytometry (see protocol). Immunoprecipitation. Immunohistochemistry on frozen sections. Functional assays.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Protocole
    FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 0. 5-1. 0 µg* of ABIN114228. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (FITC Goat anti-rat IgG (H+L)) at a 1/500 dilution. 9. Incubate the tubes at 4°C for 30-60 minutes. (It is recommended that the tubes are protected from light since most fluorochromes arelight sensitive). 10. Wash 2 times at 4°C in media B. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls).
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    PBS buffer with 0.02 % sodium azide as preservative
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid repeated freezing and thawing.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Antigène
    ITGA4 (Integrin alpha 4 (ITGA4))
    Autre désignation
    CD49d / ITGA4 (ITGA4 Produits)
    Synonymes
    anticorps CD49D, anticorps IA4, anticorps LOC100219853, anticorps integrin subunit alpha 4, anticorps integrin alpha 4, anticorps integrin subunit alpha 4 S homeolog, anticorps ITGA4, anticorps Itga4, anticorps itga4.S
    Sujet
    Alpha 4 integrin, which helps to mediate cell-cell and cell-matrix interactions. It combines with beta 1 and beta 7integrin to form VLA-4 and LPAM-1 (Peyers patch homing receptor) respectively. VLA-4 is expressed on most peripheral lymphocytes, thymocytes and monocytes. LPAM-1 is found on peripheral lymphocytes, but few thymocytes. Fibronectin and VCAM-1 act as ligands for both VLA-4 and LPAM-1. LPAM-1 also binds the mucosal vascular addressin MAdCAM-1. (1)Synonyms: CD49 antigen-like family member D, Integrin alpha-4, Integrin alpha-IV, VLA-4, VLA4
    ID gène
    16401
    NCBI Accession
    NP_034706
    UniProt
    Q00651
    Pathways
    Integrin Complex
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