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L-Selectin anticorps

SELL Reactivité: Souris FACS, IP, IHC (fro), Func Hôte: Rat Monoclonal MEL-14 unconjugated
N° du produit ABIN114230
  • Antigène Voir toutes L-Selectin (SELL) Anticorps
    L-Selectin (SELL) (Selectin L (SELL))
    Reactivité
    • 99
    • 76
    • 41
    • 29
    • 9
    • 6
    • 4
    • 3
    • 2
    • 2
    • 1
    Souris
    Hôte
    • 96
    • 67
    • 49
    Rat
    Clonalité
    • 159
    • 48
    • 3
    Monoclonal
    Conjugué
    • 73
    • 28
    • 23
    • 16
    • 13
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp L-Selectin est non-conjugé
    Application
    • 157
    • 56
    • 56
    • 53
    • 35
    • 31
    • 17
    • 13
    • 12
    • 10
    • 8
    • 7
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)
    Specificité
    This monoclonal antibody reacts with a 90 kDa protein which is involved with the homing of lymphocytes to peripheral lymph nodes.
    Purification
    Protein G Chromatography
    Stérilité
    0.2 μm filtered
    Immunogène
    Mouse B cell Lymphoma, 38C-14 Donor: Fischer Rat Spleen Fusion Partner: P3 X 63Ag8.653
    Clone
    MEL-14
    Isotype
    IgG2a
    Top Product
    Discover our top product SELL Anticorps primaire
  • Indications d'application
    Flow cytometry. Immunohistochemistry. Immunoprecipitation.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Protocole
    FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 0. 1-0. 2 µg* of this Ab. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (FITC Goat anti-rat IgG (H+L)) at 1: 500 dilution. 9. Incubate the tubes at 4°C for 30-60 minutes. (It is recommended that the tubes areprotected from light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C in media B. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results - Tissue Distribution: Mouse Strain: BALB/cCell Concentration: 1x10e6 cells per testAntibody Concentration Used: 0. 2 µg/10e6 cellsIsotypic Control: Rat IgG2aCell Source: Percentage of cells stained above controlThymus: 85. 8%Spleen: 41. 0%Lymph Node: 75. 0%Results - Strain Distribution:
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    PBS, no preservative, 0.2 μm filtered
    Agent conservateur
    Without preservative
    Conseil sur la manipulation
    Avoid repeated freezing and thawing.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Antigène
    L-Selectin (SELL) (Selectin L (SELL))
    Autre désignation
    CD62L / L-Selectin (SELL Produits)
    Synonymes
    anticorps CD62L, anticorps LAM1, anticorps LECAM1, anticorps LEU8, anticorps LNHR, anticorps LSEL, anticorps LYAM1, anticorps PLNHR, anticorps TQ1, anticorps A.11, anticorps L-selectin, anticorps LECAM-1, anticorps SELE, anticorps AI528707, anticorps Lnhr, anticorps Ly-22, anticorps Ly-m22, anticorps Lyam-1, anticorps Lyam1, anticorps selectin L, anticorps selectin, lymphocyte, anticorps SELL, anticorps Sell
    Classe de substances
    Chemical
    Sujet
    L-selectin is expressed on most T and B lymphocytes, neutrophils, monocytes, eosinophils.1 Pre-incubation of lymphocytes with this antibody completely and specifically blocks binding of lymphocytes to high endothelial venules (HEV) in vitro2,3,6 and the migration of lymphocytes to lymph nodes in vivo.2,3 Polymorphonuclear cells preincubated with this antibody do not migrate to the inflammatory foci.Synonyms: CD62 antigen-like family member L, LAM-1, LECAM1, LNHR, LYAM1, Leu-8, Leukocyte adhesion molecule 1, Leukocyte surface antigen Leu-8, Leukocyte-endothelial cell adhesion molecule 1, Lymph node homing receptor, SELL, TQ1, gp90-MEL
    ID gène
    20343
    NCBI Accession
    NP_035476
    UniProt
    P18337
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