TCR alpha/beta anticorps (Biotin)

N° du produit ABIN114324

Détail du produit anti-TCR alpha/beta anticorps (Biotin)

Antigène: TCR alpha/beta (T Cell Receptor alpha/beta (TCR alpha/beta))

Reactivité: Souris

Hôte: Hamster

Clonalité: Monoclonal

Conjugué: Cet anticorp TCR alpha/beta est conjugé à/à la Biotin

Application: Flow Cytometry (FACS)

Purification: Protein G Chromatography

Immunogène: Affinity Purified D0-11.10 TCR Donor: Armenian Hamster Fusion Partner: Murine myeloma variant P3X63Ag.653

Clone: H57-597

Isotype: IgG

Information d'application

Indications d'application: Flow cytometry. Immunoprecipitation. This clone has also been reported to work with frozen sections and Western Blotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M, cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add 1. 0 µg* of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (Streptavidin-FITC) at a 1: 500 dilution. 9. Incubate tubes at 4°C for 30 - 60 minutes (It is recommended that tubes are protectedfrom light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results - Tissue Distribution by Flow Cytometry Analysis: Mouse Strain: BALB/cCell Concentration: 1x10e6 cells per testsAntibody Concentration Used: 1. 0 µg/10e6 cellsIsotypic Control: Biotin Hamster IgGCell Source Percentage of cells stained above control: Thymus: 62. 6% see FIGURE 1Splenic T Cells: 76. 2%Strain Distribution by Flow Cytometry Analysis: Cell Concentration: 1x10e6 cells per testsAntibody Concentration Used: 1. 0 µg/106 cellsStrains Tested: BALB/c, C57BL/6, CBA/J, C3H/He, AKRPositive: BALB/c, C57BL/6, CBA/J, C3H/He, AKRNegative: none

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL

Buffer: PBS,0.02 % NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur TCR alpha/beta

Antigène: TCR alpha/beta (T Cell Receptor alpha/beta (TCR alpha/beta))

Autre désignation: T Cell Receptor / TCR alpha/beta (TCR alpha/beta Produits)

Sujet: Synonyms: T-Cell Receptor alpha, T-Cell Receptor alpha beta, T-Cell Receptor beta, TCRA, TCRB