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CD44 anticorps (FITC)

CD44 Reactivité: Rat FACS, IHC (p), IHC (fro) Hôte: Souris Monoclonal OX-49 FITC
N° du produit ABIN114364
  • Antigène Voir toutes CD44 Anticorps
    CD44
    Reactivité
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    • 81
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    Rat
    Hôte
    • 172
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    • 95
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    Souris
    Clonalité
    • 275
    • 116
    • 2
    Monoclonal
    Conjugué
    • 172
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    Cet anticorp CD44 est conjugé à/à la FITC
    Application
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    Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificité
    This anti-Rat CD44 monoclonal antibody recognizes an epitope on both standard CD44 and its splice variant.
    Réactivité croisée (Details)
    Species reactivity (tested):Rat
    Purification
    Protein G affinity chromatography
    Immunogène
    T cell blasts.
    Clone
    OX-49
    Isotype
    IgG2a
    Top Product
    Discover our top product CD44 Anticorps primaire
  • Indications d'application
    Suitable for Flow cytometry (See protocol) and Immunohistochemistry on frozen andparaffin sections.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Protocole
    FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Rat cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 1. 0-0. 5 µg* of ABIN114364 or ABIN114364X. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes are protected from light since most fluorochromes arelight sensitive). 7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results-Tissue Distribution: Rat Strain: WistarCell Concentration: 1 x 10e6 cells per testsAntibody Concentration Used: 1. 0 µg/10e6 cellsIsotypic Control: FITC Mouse IgG2a. Cell Source Percentage of cells stained above control: Thymus: 99. 6%Spleen: 75. 9%Lymph Node: 96. 8%N. B. Appropriate control samples should always be included in any labelling studies. * For optimal results in various applications, it is recommended that each investigatordetermine dilutions appropriate for individual use.
    Restrictions
    For Research Use only
  • Concentration
    0.1 mg/mL
    Buffer
    PBS with 0.02 % sodium azide as preservative and EIA grade BSA as a stabilizer
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid repeated freezing and thawing.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Antigène
    CD44
    Autre désignation
    CD44 (CD44 Produits)
    Synonymes
    anticorps CDW44, anticorps CSPG8, anticorps ECMR-III, anticorps HCELL, anticorps HUTCH-I, anticorps IN, anticorps LHR, anticorps MC56, anticorps MDU2, anticorps MDU3, anticorps MIC4, anticorps Pgp1, anticorps AU023126, anticorps AW121933, anticorps AW146109, anticorps HERMES, anticorps Ly-24, anticorps Pgp-1, anticorps CD44A, anticorps METAA, anticorps RHAMM, anticorps XCD44, anticorps cd44, anticorps cdw44, anticorps cspg8, anticorps ecmr-iii, anticorps hcell, anticorps lhr, anticorps mc56, anticorps mdu2, anticorps mdu3, anticorps mic4, anticorps mutch-i, anticorps pgp1, anticorps CD44 molecule (Indian blood group), anticorps syndecan 4, anticorps CD44 antigen, anticorps CD44 molecule (Indian blood group) S homeolog, anticorps CD44, anticorps SDC4, anticorps Cd44, anticorps cd44.S
    Sujet
    This antigen is expressed on most leukocytes (except a sub population of B cells) and increases upon activation. This antibody binds extracellularly to the standard (S) form on rat leukocytes, but it is not known if they bind to the N-terminal region. It has also been reported that the antibody may bind to melanoma cell lines that express CD44V (splice variant form). CD44 is expressed on most leukocytes except a sub population of B cells. Its expression is increased on T and B blasts.Synonyms: CDw44, ECMR-III, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, LHR, MDU2, MDU3, MIC4, PGP-1, Phagocytic glycoprotein 1
    ID gène
    10116
    UniProt
    P26051
    Pathways
    Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling
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