BRCA1 anticorps (C-Term)

N° du produit ABIN115263

Détail du produit anti-BRCA1 anticorps

Antigène: BRCA1 (Breast Cancer 1 (BRCA1))

Épitope: AA 1839-1863, C-Term

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp BRCA1 est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: BRCA1 recognizes a 22 kDa protein. This antibody stains 94 % of ovarian carcinomas with a mutation in exon 11. However, staining was absent in 100 % of tumors with mutations other than exon 11.
Cellular Localization: Cytoplasmic.

Purification: None

Immunogène: Peptide corresponding to amino acids 1839-1863 of the c-terminus of BRCA1

Clone: GLK-2

Isotype: IgM

Information d'application

Indications d'application: Immunohistochemistry on frozen and paraffin sections (ABC method: 1/15 - 1/50, Formalinfixed paraffin embedded tissue sections require high temperature antigen unmasking with10 mM citrate buffer, pH 6.0 prior to immunostaining, Incubation period of 30-60 minutesat room temperature). Use ovarian carcinoma as positive control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Tissue culture supernatant with 0.05 % sodium azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur BRCA1

Antigène: BRCA1 (Breast Cancer 1 (BRCA1))

Autre désignation: BRCA1 / RNF53 (BRCA1 Produits)

Synonymes: anticorps BRCAI, anticorps BRCC1, anticorps BROVCA1, anticorps IRIS, anticorps PNCA4, anticorps PPP1R53, anticorps PSCP, anticorps RNF53, anticorps iris, anticorps pscp, anticorps brcai, anticorps brcc1, anticorps rnf53, anticorps brca1, anticorps BRCA1, anticorps LOC100224649, anticorps BRCA1, DNA repair associated, anticorps breast cancer 1, anticorps breast cancer 1, early onset, anticorps breast cancer 1 L homeolog, anticorps BRCA1, anticorps brca1, anticorps LOC755086, anticorps Brca1, anticorps brca1.L

Sujet: BRCA1 (breast and ovarian cancer susceptibility protein 1) is a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor. It combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC (for BRCA1 associated genome surveillance complex). BRCA1 associates with RNA polymerase II, and through the C terminal domain, also interacts with histone deacetylase complex. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in the BRCA1 gene are responsible for approximately 40 % of inherited breast cancers and more than 80 % of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants have been described for this gene but only some have had their full length natures identified.Synonyms: Breast cancer type 1 susceptibility protein, RING finger protein 53

ID gène: 672

NCBI Accession: NP_009225

UniProt: P38398

Pathways: Cycle Cellulaire, Réparation de l'ADN, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus