This purified polyclonal antibody reacts with yeast Rub1. This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
Purification
Multi-step process.
Immunogène
This purified antibody was prepared from rabbit serum after repeated immunizations with full-length recombinant yeast Rub1 protein.
NEDD8
Reactivité: Humain
WB, IHC, ELISA
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Suitable for Immunoblotting (Western Blot: 1/500-1/2,000) and ELISA (1/1,000-1/5,000). Although not tested, this antibody is likely functional in Immunohistochemistry andImmunoprecipitation. A 6 kDa band corresponding to yeast Rub1 is detected. Most yeast cell lysates can be used as a positive control without induction or stimulation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore with 0.5 mL of deionized water (or equivalent).
Concentration
1.0 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing. Dilute only prior to immediate use
Stock
4 °C/-20 °C
Stockage commentaire
Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg12, Hub1. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not proteolytically processed or conjugated to other proteins. Rub1 and the corresponding human homolog Nedd8 are activated by the E1 ubiquitin-activating enzyme UBA2, that forms isopeptide linkages between thio esters. Nedd8 shows 80 % homology to ubiquitin. The best known targets of Rub1 modification are members of the cullin family. Cullins are subunits of an E3-ubiquitin ligase complex called the Skp1-/Cul1/Cdc53-F-box (SCF). The SCF promotes transfer of ubiquitin from a ubiquitin conjugating enzyme (E2) to the target protein. Rub1 modification may regulate SCF function or localization.Synonyms: NEDD8-like protein RUB1, Ubiquitin-like protein RUB1, YDR139C