LCP2 anticorps (Alexa Fluor 647)

N° du produit ABIN1176934

Détail du produit anti-LCP2 anticorps (Alexa Fluor 647)

Antigène: LCP2 (Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp LCP2 est conjugé à/à la Alexa Fluor 647

Application: Intracellular Staining (ICS)

Marque: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human SLP-76 SH2 domain Recombinant Protein

Clone: H3

Isotype: IgG2a kappa

Information d'application

Volume d'échantillon: 20 μL

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-LCP2 (Alexa Fluor 647) (ABIN1176934)

Wu, Koretzky: "The SLP-76 family of adapter proteins." dans: Seminars in immunology, Vol. 16, Issue 6, pp. 379-93, (2004) (PubMed).

Bonilla, Fujita, Pivniouk, Chan, Geha: "Adapter proteins SLP-76 and BLNK both are expressed by murine macrophages and are linked to signaling via Fcgamma receptors I and II/III." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 4, pp. 1725-30, (2000) (PubMed).

Détails sur LCP2

Antigène: LCP2 (Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2))

Autre désignation: SLP-76 (LCP2 Produits)

Synonymes: anticorps AI323664, anticorps BB161688, anticorps SLP-76, anticorps SLP76, anticorps twm, anticorps Slp76, anticorps lymphocyte cytosolic protein 2, anticorps Lcp2, anticorps LCP2

Sujet: SLP-76 (SH2 domain-containing Leukocyte Protein of 76 kDa) is a tyrosine phosphoprotein that is involved in the T cell receptor (TCR) -mediated intracellular signaling pathway. It may be involved in the signaling pathways of other peripheral blood leukocytes, thymic/splenic cells, and in human T, B, and monocytic cell lines. SLP-76 consists of several motifs that signify its importance in protein-protein interactions involved in intracellular signaling pathways, such as the SH2 domain in the C-terminus, the three amino-terminus 17-amino acid repeats with conserved tyrosine and acidic residues (DYE(S/P)P), and a proline rich region. SLP-76 has been shown to associate with Gads, Grb2, PLCg1, SLAP-130, and Vav, all of which are part of the signaling cascade in T lymphocytes. An early event in the T cell activation pathway is the phosphorylation, by the Syk-family kinase ZAP-70, of SLP-76 at the three conserved tyrosine motifs, which then mediate interactions with downstream effectors. The H3 monoclonal antibody recognizes SLP-76, regardless of phosphorylation status. This antibody conjugate is routinely tested by intracellular flow cytometry.

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway