TEK anticorps (pTyr1102) (Alexa Fluor 488)

N° du produit ABIN1177220

Détail du produit anti-TEK anticorps (Alexa Fluor 488)

Antigène: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Épitope: pTyr1102

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TEK est conjugé à/à la Alexa Fluor 488

Application: Intracellular Staining (ICS)

Marque: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Phosphorylated Human TIE2 Peptide

Clone: K93-754

Isotype: IgG2a kappa

Information d'application

Indications d'application: Either BD Cytofix™ fixation buffer or BD™ Phosflow Fix Buffer I may be used for cell fixation.

Volume d'échantillon: 20 μL

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.

Références pour anticorps anti-TEK (Alexa Fluor 488) (ABIN1177220)

Audero, Cascone, Maniero, Napione, Arese, Lanfrancone, Bussolino: "Adaptor ShcA protein binds tyrosine kinase Tie2 receptor and regulates migration and sprouting but not survival of endothelial cells." dans: The Journal of biological chemistry, Vol. 279, Issue 13, pp. 13224-33, (2004) (PubMed).

DeBusk, Hallahan, Lin: "Akt is a major angiogenic mediator downstream of the Ang1/Tie2 signaling pathway." dans: Experimental cell research, Vol. 298, Issue 1, pp. 167-77, (2004) (PubMed).

Peters, Kontos, Lin, Wong, Rao, Huang, Dewhirst, Sankar: "Functional significance of Tie2 signaling in the adult vasculature." dans: Recent progress in hormone research, Vol. 59, pp. 51-71, (2004) (PubMed).

Jones, Iljin, Dumont, Alitalo: "Tie receptors: new modulators of angiogenic and lymphangiogenic responses." dans: Nature reviews. Molecular cell biology, Vol. 2, Issue 4, pp. 257-67, (2001) (PubMed).

Murray, Padrique, Pinko, McTigue: "Mechanistic effects of autophosphorylation on receptor tyrosine kinase catalysis: enzymatic characterization of Tie2 and phospho-Tie2." dans: Biochemistry, Vol. 40, Issue 34, pp. 10243-53, (2001) (PubMed).

Edgell, McDonald, Graham: "Permanent cell line expressing human factor VIII-related antigen established by hybridization." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 80, Issue 12, pp. 3734-7, (1983) (PubMed).

Détails sur TEK

Antigène: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Autre désignation: TIE2 (TEK Produits)

Synonymes: anticorps TEK, anticorps DKFZp469E064, anticorps AA517024, anticorps Cd202b, anticorps Hyk, anticorps Tie2, anticorps tie-2, anticorps CD202B, anticorps TIE-2, anticorps TIE2, anticorps VMCM, anticorps VMCM1, anticorps Tie-2, anticorps tie2, anticorps TEK receptor tyrosine kinase, anticorps angiopoietin-1 receptor, anticorps TEK tyrosine kinase, endothelial, anticorps TEK, anticorps LOC100408334, anticorps tek, anticorps Tek

Sujet: TIE2 (Tyrosine kinase with Immunoglobulin-like and EGF-like domains 2), also known as TEK (Tunical Endothelial Kinase), is an endothelial and hematopoietic cell-specific receptor tyrosine kinase (RTK) that is critical to the development and maintenance of the vasculature and highly conserved among vertebrate species. The angiopoietins are ligands of TIE2, and the abnormal vascular growth that often occurs in solid tumors is a result of disruptions in the coordinated actions of the angiopoietins, TIE2, and the closely related TIE1 RTK. Upon activation by angiopoietins, TIE2 autophosphorylates at least 2 tyrosines in its protein kinase domain and at least 3 tyrosines in its C-terminal tail. Phosphorylation at tyrosine 1102 (Y1102) leads to activation of the Akt signal transduction pathway, enhancing endothelial cell survival. The phosphorylated Y1102 also recruits and activates the src homology 2 domain-containing adaptor proteins Grb2 and ShcA, affecting endothelial cell adhesion and motility. The K93-754 monoclonal antibody recognizes the phosphorylated Y1102 in the C-terminal tail of activated TIE2. The orthologous phosphorylation site in mouse TIE2 is Y1100.
Synonyms: TEK, VMCM, CD202b

Pathways: Signalisation RTK, Growth Factor Binding