MTOR anticorps (pSer2448)

N° du produit ABIN117909

Détail du produit anti-MTOR anticorps

Antigène: MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))

Épitope: AA 2440-2457, pSer2448

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MTOR est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificité: Reactivity occurs with phosphorylated mTOR from Human derived tissues and cells. Reactivity against mTOR from other species has not been determined, however, reactivity with Mouse and Rat is suggested based on protein sequence homologies.

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography using the immunizing peptide after immobilization to a solid phase.

Immunogène: A synthetic peptide corresponding to amino acids 2440-2457 of Human mTOR

Information d'application

Indications d'application: This antibody has been tested for use in Immunohistochemistry (5.0 μg/mL), ELISA(1/15,000-1/65,000) and Western blotting (1/500-1/2,000). Western blotting shows reactivity specific for phospho mTOR detecting a band atapproximately 250 kDa.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.88 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, containing 0.01 % (w/v) Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Détails sur MTOR

Antigène: MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))

Autre désignation: mTOR / FRAP1 (mTOR Produits)

Synonymes: anticorps FRAP1, anticorps FRAP, anticorps FRAP2, anticorps RAFT1, anticorps RAPT1, anticorps 2610315D21Rik, anticorps AI327068, anticorps Frap1, anticorps flat, anticorps frap1, anticorps tor, anticorps wu:fc22h08, anticorps mechanistic target of rapamycin kinase, anticorps mechanistic target of rapamycin, anticorps mechanistic target of rapamycin (serine/threonine kinase), anticorps MTOR, anticorps Mtor, anticorps mtor

Sujet: Mammalian target of rapamycin (mTOR) is a serine and threonine protein kinase that regulates numerous cellular functions, in particular, the initiation of protein translation. Rapamycin is a natural product macrolide that induces G1 growth arrest in yeast, Drosophila, and mammalian cells. mTOR is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, mTOR is a 245-kD protein referred to as RAFT1 with significant homology to the Saccharomyces cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of mTOR to FKBP12-rapamycin correlates with the ability of these ligands to inhibit cell cycle progression.Synonyms: FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, FRAP, FRAP2, Mammalian target of rapamycin, RAPT1, Rapamycin target protein, Serine/threonine-protein kinase mTOR, TOR

ID gène: 2475

NCBI Accession: NP_004949

UniProt: P42345

Pathways: Signalisation PI3K-Akt, Signalisation RTK, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, BCR Signaling, L'effet Warburg