RBX1 anticorps (C-Term)

N° du produit ABIN117957

Détail du produit anti-RBX1 anticorps

Antigène: RBX1 (Ring-Box 1, E3 Ubiquitin Protein Ligase (RBX1))

Épitope: AA 94-108, C-Term

Reactivité: Humain, Souris, Poisson zèbre (Danio rerio), C. elegans

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RBX1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Séquence: Human ROC1 (108 aa 12.2 kDa) : 1 maaamdvdtp sgtnsgagkk rfevkkwnav alwawdivvd ncaicrnhim dlciecqanq 61 asatseectv awgvcnhafh fhcisrwlkt rqvcpldnre wefqkygh

Specificité: This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human, mouse, C.elgans and zebra fish ROC1. Cross reactivity may also occur with ROC1 from other sources. Sufficient sequence differences exist to suggest that this antibody would not react with other RING box proteins such as ROC2 and APC11.

Purification: Delipidation and defibrination.

Immunogène: Prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 94-108 of Human ROC1 (C-terminal) coupled to KLH.

Information d'application

Indications d'application: This antibody reacts with human ROC1 by Western blot (1: 500-1: 1,000) andImmunoprecipitation. The antibody immunoprecipitates in vitro translated protein andprotein from overexpressing cell lysates (using HeLa and NIH-3T3, and others). Coimmunoprecipitation of related cullin proteins does occur. Coimmunoprecipitation ofubiquitin ligase activity will also occur. A 12.2 kDa band corresponding to human ROC1 isdetected. Most cell lines expressing ROC1 can be used as a positive control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 85 mg/mL (by Refractometry)

Stock: 4 °C

Stockage commentaire: Store undiluted at 2-8 °C.

Détails sur RBX1

Antigène: RBX1 (Ring-Box 1, E3 Ubiquitin Protein Ligase (RBX1))

Autre désignation: RBX1 / RNF75 (RBX1 Produits)

Synonymes: anticorps BA554C12.1, anticorps RNF75, anticorps ROC1, anticorps 1500002P15Rik, anticorps AA517855, anticorps im:7137515, anticorps zgc:136444, anticorps RBX1, anticorps ATRBX1, anticorps F7C8.160, anticorps F7C8_160, anticorps HRT1, anticorps REGULATOR OF CULLINS-1, anticorps RING-BOX 1, anticorps RING-box 1, anticorps hop21, anticorps BEST:CK01110, anticorps CG16982, anticorps CK01110, anticorps Dmel\CG16982, anticorps EG:115C2.11, anticorps Rbx1, anticorps Rbx1/Roc, anticorps Roc, anticorps Roc1alpha, anticorps anon-EST:Posey65, anticorps dRbx1, anticorps dRoc1, anticorps dRoc1a, anticorps ring-box 1, anticorps ring-box 1, E3 ubiquitin protein ligase, anticorps RING-box 1, anticorps RING-box protein 1, anticorps RING-box protein 1a, anticorps Regulator of cullins 1a, anticorps RBX1, anticorps Rbx1, anticorps rbx1, anticorps MGG_08844, anticorps LOC9308017, anticorps rbx-1, anticorps Roc1a

Sujet: ROC1 also known as RING-box protein 1, Rbx1, Regulator of cullins 1, RING finger protein 75, and ZYP protein, is a component of the SCF (SKP1-CUL1-F-box protein) and the CBC(VHL) (CUL2-elonging BC-VHL) E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. ROC1 appears to recruit the E2 ubiquitination enzyme through the RING-type zinc finger in a manner similar to CDC34, and brings it into close proximity to the substrate. The RING-type zinc finger domain is essential for ubiquitin ligase activity. ROC1 probably also stimulates CDC34 autoubiquitination and promotes the neddylation of CUL1 and probably CUL2. ROC1 has a cytoplasmic and nuclear localization and is widely expressed in most tissues.Synonyms: E3 ubiquitin-protein ligase RBX1, Protein ZYP, RING finger protein 75, RING-box protein 1, ROC1, Regulator of cullins 1

ID gène: 9978

NCBI Accession: NP_055063

UniProt: P62877

Pathways: Cycle Cellulaire, M Phase, SARS-CoV-2 Protein Interactome