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MIF anticorps (AA 2-32)

MIF Reactivité: Humain WB, EIA Hôte: Poulet Polyclonal unconjugated
N° du produit ABIN118028
  • Antigène Voir toutes MIF Anticorps
    MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))
    Épitope
    • 18
    • 10
    • 7
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 2-32
    Reactivité
    • 81
    • 37
    • 33
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Humain
    Hôte
    • 69
    • 19
    • 4
    • 2
    • 1
    • 1
    Poulet
    Clonalité
    • 63
    • 31
    Polyclonal
    Conjugué
    • 57
    • 10
    • 6
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Cet anticorp MIF est non-conjugé
    Application
    • 61
    • 43
    • 22
    • 13
    • 13
    • 11
    • 10
    • 8
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Enzyme Immunoassay (EIA)
    Séquence
    P-M-F-I-V-N-T-N-V-P-R-A-S-V-P-D-G-F-L-S-E-L-T-Q-Q-L-A-Q-A-T-G
    Specificité
    This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum. The antibody is directed against the 12,300 MW human MIF protein and is useful in determining its presence in various assays. If neutralization experiments are performed for human MIF activity in bioassays, it is recommended to incubate the sample with a 1:500 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal chicken IgG is recommended. If FACS analysis experiments are performed for human MIF caution should be exhibited as the F( c) domain of the chicken IgG molecule may interact with cells non-specifically. Endotoxin Content: < 10 pg/μl by LAL method.
    Purification
    Multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis
    Immunogène
    This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to aa 2-32 of Human MIF conjugated to keyhole limpet hemocyanin (KLH). MIF is a proinflammatory cytokine that plays an important role in systemic inflammatory events.
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    Discover our top product MIF Anticorps primaire
  • Indications d'application
    This IgY fraction antibody of anti-Human MacrophageMigration Inhibitory Factor (MIF) has been tested for use in ELISA and Immunoblotting. Although not tested, this antibody may also be useful for Neutralization assays,Immunohistochemistry and Flow Cytometry. The antibody recognizes 12,300 MW maturehuman MIF. The MIF gene encodesa protein of 115 aa. The initiating methionine is cleaved leaving a mature protein of 114 aa. Reactivity in other immunoassays is unknown. Immunoblot: Using IRDYE800 conjugated Goat-a-Chicken IgG [H&L] MX10. A workingdilution range of 1: 500-1: 1,000 is suggested for this application to detect human MIF fromsupernatants or lysates of 2 x 10^6 endotoxin-stimulated human peripheral bloodmononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1 % (v/v) human serumplus 10 ng/mL E. coli LPS. This product has been assayed by ELISA against human MIF using HRP ConjugatedGoat-a-Chicken IgG [H&L] MX10 and ABTS as a substrate for 30 minutes at roomtemperature. A working dilution range of 1: 1,000-1: 2,000 is suggested for this product. For use in ELISAformats, this antibody is best used as the second antibody in combination with amonoclonal antibody as a capture antibody.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Reconstitution
    Restore with 0.1 mL of deionized water (or equivalent).
    Concentration
    5.0 mg/mL (by UV absorbance at 280 nm)
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid repeated freezing and thawing. Dilute only prior to immediate use
    Stock
    4 °C/-20 °C
    Stockage commentaire
    Store vial at 2-8 °C prior to restoration. For extended storage mix with glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid.
  • Antigène
    MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))
    Autre désignation
    MIF (MIF Produits)
    Synonymes
    anticorps mif, anticorps Mif, anticorps gif, anticorps glif, anticorps mmif, anticorps LOC100136498, anticorps LOC100284350, anticorps LOC100284546, anticorps GIF, anticorps Glif, anticorps GLIF, anticorps MMIF, anticorps macrophage migration inhibitory factor L homeolog, anticorps macrophage migration inhibitory factor, anticorps macrophage migration inhibitory factor (glycosylation-inhibiting factor), anticorps mif.L, anticorps mif, anticorps MIF, anticorps Mif, anticorps PHATRDRAFT_49660, anticorps LOC100136498, anticorps cl405_1, anticorps LOC100284546
    Sujet
    Cytokines play an important role in inflammation and immunity. Macrophage migration inhibitory factor (MIF) was one of the first cytokine activities described and is a proinflammatory cytokine that plays an important role in systemic inflammatory events. MIF's cytokine activity was initially described as a T cell-derived factor that inhibited the random migration of macrophages, hence its name. Since it was cloned and expressed in pure form, MIF's activities have been established to play many roles in development and various disease states. For example, MIF is released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counterregulates the immunosuppressive effects of steroids on immune cell activation and cytokine production. In in vitro experiments MIF is significantly upregulated by the stimulation of lipopolysaccharide (LPS) using 10 pg/mL to 10 ng/mL of LPS and reaches the maximum 12 h after the stimulation. MIF also prevents cleavage of Bax into an 18- kDa active fragment, and, consequently, reduces activation of the critical effector caspase 3, suggesting that MIF inhibits apoptosis pathways proximal to mitochondria activation and is therefore a survival factor. And, MIF is also known to exhibit enzymatic activities. A pathologic role for MIF has been described in many conditions including arthritis, asthma, and inflammatory bowel disease, and may also play a role in the control of cell growth in certain cancers. Consequently MIF is suggested to be a potential therapeutic target for human diseases.Synonyms: GLIF, Glycosylation-inhibiting factor, MMIF, Macrophage migration inhibitory factor, Phenylpyruvate tautomerase
    ID gène
    4282
    NCBI Accession
    NP_002406
    UniProt
    P14174
    Pathways
    Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
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