Immunoaffinity Chromatography. This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads.
Suitable for Immunoblotting (1/1,000-1/4,000, Western or dot blot), ELISA (1/10,000-1/40,000), Immunoperoxidase Electron Microscopy and Immunohistochemistry (1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiringlot-to-lot consistency.
Other applications not tested. Otimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Reconstitution
Restore with 2.0 mL of deionized water (or equivalent).
Concentration
0.8 mg/mL
Buffer
0.01 M Potassium Phosphate, 0.21 M Sodium Chloride, pH 7.2 containing 15 mg/mL BSA (IgG and Protease free) as stabilizer.
Agent conservateur
Azide free
Conseil sur la manipulation
Avoid repeated freezing and thawing. Dilute only prior to immediate use Centrifuge product if not completely clear after standing at room temperature. Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Stock
4 °C/-20 °C
Stockage commentaire
Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50 % and then aliquot contents and freeze at -28 °C or below. This product is stable for one month at 2-8 °C as an undiluted liquid.