CD19 anticorps (PE)

N° du produit ABIN118162

Détail du produit anti-CD19 anticorps (PE)

Antigène: CD19 (CD19 Molecule (CD19))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD19 est conjugé à/à la PE

Application: Flow Cytometry (FACS)

Specificité: This antibody recognises the CD19 cell surface antigen, a 95 kDa glycoprotein expressed by B lymphocytes. Clone 6D5 has been shown to recognise the same epitope as clone ID3 in cross-competition assays.

Purification: Affinity chromatography on Protein G

Immunogène: Human K562 cell line transfected with murine CD19. Spleen cells from immunised rats were fused with cells of the P3X63.Ag8.653 myeloma cell line.

Clone: 6D5

Isotype: IgG2a

Information d'application

Indications d'application: Flow Cytometry: Use 10 μL of Neat-1/10 diluted antibody to label 10^6 cells in 100 μL. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 1 mL distilled water

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: This product is photosensitive and should be protected from light

Stock: 4 °C

Stockage commentaire: Prior to and following reconstitution store the antibody at 2-8 °C. DO NOT FREEZE!

Détails sur CD19

Antigène: CD19 (CD19 Molecule (CD19))

Autre désignation: CD19 (CD19 Produits)

Synonymes: anticorps B4, anticorps CVID3, anticorps AW495831, anticorps CD19, anticorps CD19 molecule, anticorps CD19 antigen, anticorps CD19, anticorps Cd19

Sujet: CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 Molecule is expressed on 100 % of the peripheral B cells as defined by expression of kappa or lamda light chains. It is expressed on approximately 10 % of normal human peripheral blood cells and approximately 60 % of splenic lymphocytes. It is not expressed on granulocytes, monocytes or T cells as defined by CD3 expression. CD19 defines a pan B antigen which is expressed from the earliest stages of B progenitor development, but is lost on terminal differentiation to plasma cells. It may also be present on some early myeloid progenitors, particularly those of the monoblastic type. The CD19 antigen is expressed on approximately 12 % of peripheral blood lymphocytes. It appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen.Synonyms: B-cell marker, B-lymphocyte surface antigen B4, Differentiation antigen CD19, Leu-12

ID gène: 12478

NCBI Accession: NP_033974

UniProt: P25918

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway