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CD34 anticorps

CD34 Reactivité: Souris FACS, IHC (p), IP Hôte: Rat Monoclonal MEC14-7 unconjugated
N° du produit ABIN119262
  • Antigène Voir toutes CD34 Anticorps
    CD34
    Reactivité
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    Hôte
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    Rat
    Clonalité
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    Monoclonal
    Conjugué
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    Cet anticorp CD34 est non-conjugé
    Application
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    Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
    Specificité
    This antibody recognises the CD34 cell surface antigen, wihich is expressed by endothelial cells and by haematopoietic stem cells. It recognises a Neuraminidase sensitive epitope. As in the human system, CD34 antibodies in the mouse demonstrate slightly different staining patterns depending on their fine specificity. Clone MEC14.7 appears to recognise a subset of the stem cell population recognised by clone RAM34, and it is thought that this is due to differences in the epitope recognised by the two antibodies.
    Purification
    Affinity Chromatography on Protein G from tissue culture supernatant.
    niveau d'endotoxine
    Low Endotoxin
    Clone
    MEC14-7
    Isotype
    IgG2a
    Top Product
    Discover our top product CD34 Anticorps primaire
  • Indications d'application
    Flow Cytometry: use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunoprecipitation.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01 EU/ug
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    PBS without preservatives.
    Agent conservateur
    Without preservative
    Conseil sur la manipulation
    Avoid repeated freezing and thawing.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
  • Antigène
    CD34
    Autre désignation
    CD34 (CD34 Produits)
    Synonymes
    anticorps AU040960, anticorps CD34 molecule, anticorps CD34 antigen, anticorps CD34, anticorps Cd34
    Sujet
    The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.Synonyms: Hematopoietic progenitor cell antigen CD34, Hematopoietic progenitor cell marker
    ID gène
    12490
    NCBI Accession
    NP_598415
    UniProt
    Q64314
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